植物保护2026，Vol.52Issue(4)：76-87,12.DOI:10.16688/j.zwbh.2025423
云南玉溪不同地区山黄麻丛枝植原体分子鉴定及多位点基因分析
Molecular identification and multilocus gene analysis of Trema levigata witches'-broom phytoplasma from different regions of Yuxi,Yunnan
摘要
Abstract
In recent years,Trema levigata witches'-broom disease has been continuously reported in different regions of Yunnan province,China.Infected plants exhibit typical witches'-broom and little leaf symptoms,causing severe damage.To confirm the pathogen of this disease,determine its taxonomic status,and clarify the phylogenetic relationships among isolates,PCR amplification of the 16S rRNA gene and ten housekeeping genes(rp,tuf,secA,secY,ipt,dnaK,fusA,gyrB,pyrG,and rpoB)was conducted for phylogenetic analysis.In addition,classification and identification of the isolates were performed using the online phytoplasma tool iPhyClassifier.The results showed that the pathogens causing T.levigata witches'-broom disease in Yuanjiang,Yimen,Xinping,and Eshan of Yunnan all belonged to'Candidatus Phytoplasma asteris'-related strains and were members of the phytoplasma 16Sr Ⅰ-Ⅹ subgroup.Phylogenetic trees based on the fusA,gyrB,and rpoB genes showed that the four isolates were closely related.Concatenated analysis of the ten housekeeping genes of the Yuanjiang isolate indicated that it was more closely related to members of the 16Sr Ⅰ-B subgroup.Therefore,the witches'-broom disease of T.levigata in the four regions was likely caused by the same phytoplasma.This study supplements the basic data on phytoplasmas and lays a solid foundation for further in-depth research on the epidemiology and control of T.levigata phytoplasma disease.关键词
植原体/山黄麻/16SrⅠ-Ⅹ/丛枝病/多位点分析Key words
phytoplasma/Trema levigata/16Sr Ⅰ-Ⅹ/witches'-broom disease/multilocus analysis分类
农业科技引用本文复制引用
王连春,马晔铭,万琼莲,李学美,乔开,赵静,虎思雨,罗秋洁,周海霞,高达,王武雄..云南玉溪不同地区山黄麻丛枝植原体分子鉴定及多位点基因分析[J].植物保护,2026,52(4):76-87,12.基金项目
云南省地方本科高校基础研究联合专项-青年项目(202101BA070001-274) （202101BA070001-274）
云南省地方本科高校基础研究联合专项-面上项目(202401BA070001-153) （202401BA070001-153）
