植物营养与肥料学报2026，Vol.32Issue(7)：1428-1440,13.DOI:10.11674/zwyf.2025484
羊粪和生物质炭调控玉米根际土壤酶活性空间分布和微生物群落结构
Sheep manure and biochar regulate spatial distribution of soil enzyme activity and microbial community structure in the maize rhizosphere
摘要
Abstract
[Objectives]This study aims to explore the mechanisms by which the addition of sheep manure and biochar drives enzyme activities and their spatial distribution characteristics in the maize rhizosphere,providing a theoretical basis for the efficient application of organic fertilizers.[Methods]A rhizobox-based maize cultivation experiment was conducted with four treatments:no fertilization(CK),sole application of sheep manure(SM),sole application of biochar(BC),and combined application of sheep manure and biochar(SM+BC).The maize was harvested after six weeks of growth.Using in situ soil zymography and high-throughput sequencing techniques,we systematically analyzed the physicochemical properties,microbial community composition,and spatial patterns of enzyme activities in the rhizosphere soil of maize.[Results]1)Compared with CK,SM and SM+BC significantly increased soil pH,total nitrogen(TN),alkali-hydrolyzable nitrogen(AN),available phosphorus(AP),and total phosphorus(TP),promoting maize aboveground and root growth;while BC had no significant effect on plant growth.2)SM and SM+BC significantly increased the activities of alkaline phosphatase(ALP),β-1,4-N-acetylglucosaminidase(NAG),and β-glucosidase(BG)in the rhizosphere soil,with increases ranging from 200.8%-589.0%for SM and 146.7%-400.8%for SM+BC,respectively.Both treatments expanded the enzyme activity hotspot area and extended the rhizosphere extent.BC had no significant effect on hydrolytic enzyme activity.3)Sheep manure enriched copiotrophic bacteria such as Proteobacteria,Bacteroidota,Actinobacteriota,and Firmicutes,as well as fungal groups such as Ascomycota,while inhibiting oligotrophic taxa such as Acidobacteriota.Microbial community composition was significantly associated with soil nutrients and enzyme activity.The structural equation model indicated that exogenous organic matter primarily improved the soil chemical environment,regulated plant traits and bacterial community structure,thereby synergistically enhancing rhizosphere enzymatic activity and expanding functional zones.[Conclusions]Sheep manure significantly increased rhizosphere soil pH and nutrient content(e.g.,nitrogen and phosphorus),enriched copiotrophic bacterial taxa including Proteobacteria,Bacteroidota,Actinobacteriota,and Firmicutes,as well as fungal groups such as Ascomycota,while suppressing oligotrophic taxa such as Acidobacteriota.It enhanced the activities of C,N,and P cycling-related enzymes(BG,NAG,and ALP),expanded enzyme activity hotspot areas,and extended the rhizosphere extent,thereby substantially promoting maize aboveground and root growth.In contrast,biochar was less effective than sheep manure in directly improving nutrient availability,exerted a lower impact on rhizosphere microorganisms and N and P metabolism-related enzyme activities,and did not significantly promote maize growth.The long-term effects of sheep manure and biochar on rhizosphere physicochemical and biological properties still require long-term experimental validation.关键词
原位土壤酶谱技术/羊粪/生物质炭/玉米/酶活性空间分布Key words
soil zymography/sheep manure/biochar/maize/spatial distribution of enzyme activity引用本文复制引用
方慧璇,杨光亚,陈俊辉,王嘉林,周燕,秦华,马晓敏..羊粪和生物质炭调控玉米根际土壤酶活性空间分布和微生物群落结构[J].植物营养与肥料学报,2026,32(7):1428-1440,13.基金项目
国家重点研发计划项目(2023YFD1901800) （2023YFD1901800）
国家自然科学基金(32371726,42307421,42407449) （32371726,42307421,42407449）
浙江省"尖兵""领雁"研发攻关计划项目(2023C02005) （2023C02005）
浙江省"三农九方"科技协作计划项目(2025SNJF018). （2025SNJF018）
