作物杂志Issue(4)：17-26,10.DOI:10.16035/j.issn.1001-7283.2026.04.003
利用 CRISPR/Cas9 技术编辑GS3、GS9 和GW2 基因改良水稻粒型
Improvement of Rice Grain Shape by Editing of GS3,GS9 and GW2 Genes Using CRISPR/Cas9 Technology
摘要
Abstract
The CRISPR/Cas9 gene-editing technique was used to simultaneously modify the grain shape-related genes GS3,GS9,and GW2 in the rice cultivar Xigong 6.The aim was to improve the rice grain shape through a multi-gene editing strategy.Knockout target sites were designed within the first exons of each gene,and CRISPR/Cas9 expression vectors were constructed to produce T0 generation transgenic plants via Agrobacterium-mediated genetic transformation.The results showed that various types of gene-editing mutations,including biallelic homozygous and double heterozygous mutations,were observed in the T0 generation.T1 generation plants were obtained through self-pollination,and mutant materials lacking exogenous transgenic components,while mutations in the GS3,GS9,and GW2 consistent with those in the T0 generation were screened.Agronomic trait analysis of T1 generation mutants revealed significant improvements in grain morphology:mutants 1188-42-1 showed a maximum grain length increase of 21.62%,1188-4-12 exhibited a 13.16%increase in grain width,and 1188-4-13 displayed a 26.65%enhancement in length-width ratio.Concurrently,1000-grain weight,grain weight per plant,yield per plant,brown rice rate,rice milling rate,head rice rate,and chalkiness degree were all significantly increased.However,a minor reduction in seed-setting rate 4.12%-13.40%was observed across all mutants.Notable variations in chalky rate were recorded:line 1188-18-8 showed a 175.00%increase,while 1188-42-2 demonstrated a 50.50%decrease.关键词
CRISPR/Cas9/水稻/粒型/多基因编辑Key words
CRISPR/Cas9/Rice/Grain shape/Multi-gene editing引用本文复制引用
阙涛,吴娴,王忠妮,吴钱蓉,龙武华,王倩,朱速松..利用 CRISPR/Cas9 技术编辑GS3、GS9 和GW2 基因改良水稻粒型[J].作物杂志,2026,(4):17-26,10.基金项目
贵州省优质特色水稻研发与转化及园区技术服务能力建设[黔科合平台人才(2017)5719 号] （2017）
贵州省农业农村厅种业资金项目"贵州优质资源优异基因挖掘及其分子改良" （）
喀斯特山区特色作物生物育种创新能力建设(黔科合服企[2024]003-1) （黔科合服企[2024]003-1）
