作物杂志Issue(4)：57-66,10.DOI:10.16035/j.issn.1001-7283.2026.04.007
利用CRISPR/Cas9 技术编辑 Badh2基因改良水稻优质恢复系 QR79 的香味
CRISPR/Cas9-Mediated Editing of the Badh2 Gene Improves Aroma in Elite Restorer Line QR79
摘要
Abstract
In this research,the elite restorer line QR79 was used as the recipient for CRISPR/Cas9-mediated editing of the badh2 gene,with two targeting sites designed within exon 1 and exon 2.The primary aim was to obtain homozygous mutant lines characterized by a distinct aroma profile,complete absence of transgenic elements,and stable inheritance of main agronomic traits.The expression level of the Badh2 gene,the concentration of 2-acetyl-1-pyrroline(2AP),the content of γ-aminobutyric acid(GABA),as well as the key agronomic traits of both the wild-type and mutant genotypes were systematically quantified and comprehensively analyzed.The results showed that,compared with the wild type,the expression levels of the Badh2 gene in the homozygous mutant plants QR79-badh2-1 and QR79-badh2-2 were significantly reduced,the content of 2AP were significantly increased,while the content of GABA were significantly decreased.At the same time,the main agronomic traits such as grain length,1000-grain weight,head rice rate,and the seed-setting rate remained stable,but the yield components were affected to a certain extent.In conclusion,the genetic improvement of the aroma characteristics of the high-quality rice restorer line QR79 was successfully achieved through the CRISPR/Cas9 gene editing technology,which significantly increased the content of the aroma component 2AP,provided theoretical support and genetic resources for accelerating the research and development of new fragrant indica rice varieties.关键词
香稻/Badh2/CRISPR/Cas9/2-乙酰-1-吡咯啉/γ-氨基丁酸Key words
Fragrant rice/Badh2/CRISPR/Cas9/2-acetyl-1-pyrroline/γ-aminobutyric acid引用本文复制引用
吴钱蓉,吴娴,姜雪,王倩,宫彦龙,王忠妮,朱速松,宋莉..利用CRISPR/Cas9 技术编辑 Badh2基因改良水稻优质恢复系 QR79 的香味[J].作物杂志,2026,(4):57-66,10.基金项目
贵州省农业科学院青年基金项目[黔农科青年基金(2023)30] （2023）
贵州省科技计划项目(课题)[黔科合基础-ZK(2022)一般284] （课题）
贵州省优质特色水稻研发与转化及园区技术服务能力建设[黔科合平台人才(2017)5719号] （2017）
