中国现代中药2026，Vol.28Issue(7)：1312-1322,11.DOI:10.13313/j.issn.1673-4890.20260105002
不同产区甘草药材遗传特征与品质关键驱动因子研究
Genetic Characteristics and Key Driving Factors of Quality of Glycyrrhizae Radix et Rhizoma from Different Production Areas
摘要
Abstract
Objective:To clarify the botanical origin and population genetic diversity of Glycyrrhizae Radix et Rhizoma from different production areas and analyze the key factors affecting the accumulation of active components,thereby providing a basis for standardized cultivation and quality control of Glycyrrhizae Radix et Rhizoma.Methods:Inter-simple sequence repeat(ISSR)molecular markers were employed to analyze the genetic characteristics of the samples.DNA barcoding was used for the identification of botanical origin.The content of eight active components in Glycyrrhizae Radix et Rhizoma was determined by high performance liquid chromatography(HPLC).The data of 19 climatic factors were obtained from the Geographic Information System for Global Medicinal Plants(GMPGIS).Mantel correlation analysis and grey relational analysis were performed to reveal the effects of genetic factors and climatic factors on the accumulation of active components in Glycyrrhizae Radix et Rhizoma from different botanical origins.Results:The tested samples belonged to two species:Glycyrrhiza uralensis and G.glabra.The populations from Gansu and Inner Mongolia exhibited rich genetic diversity.Climatic factors such as temperature and precipitation,as well as genetic parameters including the number of alleles and gene diversity index,showed positive correlations with the content of active components in Glycyrrhizae Radix et Rhizoma.Climatic factors were the dominant factors affecting the accumulation of active components in G.uralensis,whereas genetic factors mainly influenced the accumulation of active components in G.glabra.Conclusion:This study clarifies the botanical origin of Glycyrrhizae Radix et Rhizoma from different production areas and the effects of genetic and climatic factors on the active component content of the two species,providing a methodological reference for quality evaluation and geoherbalism research of Glycyrrhizae Radix et Rhizoma.关键词
甘草/DNA条形码/简单重复区间序列/相关性分析/灰色关联度分析Key words
Glycyrrhizae Radix et Rhizoma/DNA barcoding/inter-simple sequence repeat(ISSR)/correlation analysis/gray correlation analysis分类
医药卫生引用本文复制引用
刘丽,徐佳,王婷,张乃嘦,周豫新,张伟,徐福荣,董林林..不同产区甘草药材遗传特征与品质关键驱动因子研究[J].中国现代中药,2026,28(7):1312-1322,11.基金项目
国家重点研发计划项目(2022YFC3501804) （2022YFC3501804）
北京市科技新星计划项目(20240484546) （20240484546）
