中国现代中药2026，Vol.28Issue(7)：1343-1351,9.DOI:10.13313/j.issn.1673-4890.20250617004
基于响应面法对藏药线叶龙胆不同部位中齐墩果酸和熊果酸的提取方法研究
Study on Extraction Methods of Oleanolic Acid and Ursolic Acid from Different Parts of Tibetan Medicine Gentiana Lawrencei Based on Response Surface Methodology
摘要
Abstract
Objective:This paper aims to establish a high-performance liquid chromatography(HPLC)method for determining the contents of oleanolic acid and ursolic acid in the Tibetan medicine Gentiana lawrencei var.farreri,optimize the extraction process,and provide scientific evidence for the quality evaluation and rational development and utilization of the medicine.Methods:The extraction process of oleanolic acid and ursolic acid from G.lawrencei var.farreri was optimized by Box-Behnken design response surface methodology,and the factors such as drug-liquid ratio,extraction temperature,and ultrasonic time were optimized.An Agilent Zorbax XDB-C18 chromatographic column(250 mm×4.6 mm,5 μm)was used with the mobile phase of methanol and 0.05 mol·L-1 ammonium acetate solution(88∶12)at a flow rate of 0.6 mL·min-1,a column temperature of 25℃,a detection wavelength of 215 nm,and an injection volume of 10 μL.Results:When methanol was used as the extraction solvent,the extraction efficiency was the highest at a solid-liquid ratio of 1∶40,an extraction temperature of 29℃,and an ultrasonic time of 24 min.Both components achieved baseline separation under the above chromatographic conditions.The linear equations for oleanolic acid and ursolic acid were Y=516 027X-25.425(r=0.999 9)and Y=489 438X-48.954(r=0.999 8),respectively.The average recoveries were 103.30%(RSD=0.81%)for oleanolic acid and 99.52%(RSD=2.43%)for ursolic acid.The results of comparison of oleanolic acid and ursolic acid contents in different parts of G.lawrencei var.farreri showed that the flower exhibits the highest mass fraction(23.302 mg·g-1),followed by stems and leaves(18.419 mg·g-1)and the whole herb(18.457 mg·g-1),and that the contents of the latter two were similar.Conclusions:The extraction process of G.lawrencei var.farreri optimized via response surface methodology is stable and reliable,and it is suitable for the extraction of oleanolic acid and ursolic acid from G.lawrencei var.farreri.At the same time,the contents of oleanolic acid and ursolic acid in G.lawrencei var.farreri were determined by the HPLC method.The method is accurate,rapid,and stable,and it can be used for the quality control of G.lawrencei var.farreri.关键词
线叶龙胆/高效液相色谱法/响应面优化/齐墩果酸/熊果酸/含量测定Key words
Gentiana lawrencei var.farreri(Balf.f.)T.N.Ho/high-performance liquid chromatography/response surface optimization/oleanolic acid/ursolic acid/content determination分类
医药卫生引用本文复制引用
王圆圆,林丽,王振恒,席少阳,晋玲..基于响应面法对藏药线叶龙胆不同部位中齐墩果酸和熊果酸的提取方法研究[J].中国现代中药,2026,28(7):1343-1351,9.基金项目
中央本级重大增减支项目(2060302) （2060302）
中国工程院战略研究与咨询项目(GS2021ZDA06) （GS2021ZDA06）
道地药材生态种植及质量保障项目(国中医药科技[2020]153号) （国中医药科技[2020]153号）
财政部和农业农村部:国家现代农业产业技术体系资助项目 （）
甘肃省高校教师创新基金项目(2025-A116) （2025-A116）
