中药与临床2026，Vol.17Issue(4)：35-43,中插10-中插11,11.
基于网络药理学、分子对接和实验验证索罗西汤颗粒预防慢性阻塞性肺疾病的作用及机制研究
Study on the mechanism of Srolo Bzhtang in the prevention of chronic obstructive pulmonary disease based on net-work pharmacology,molecular docking and in vivo experiments
摘要
Abstract
Objective:To preliminarily investigate the pharmacodynamics and mechanism of Tibet medicine classic formula Srolo Bzhtang(SBT)granules in preventing chronic obstructive pulmonary disease(COPD)based on network pharmacology,molecular dock-ing,and in vivo experiments.Methods:The intersection of SBT blood components and COPD disease targets was screened,followed by network construction and enrichment analysis.Key targets were validated using molecular docking.Rats were divided into blank control group,model group,dexamethasone group,and SBT low-,medium-,and high-dose groups.The COPD model was established using cigarette smoke combined with lipopolysaccharide in all groups except the control group.Preventive administration was adopted,with the dexamethasone group(0.2 g·kg-1),SBT low-dose group(1.35 g·kg-1),SBT medium-dose group(2.7 g·kg-1),and SBT high-dose group(8.1 g·kg-1),each consisting of 10 rats.All groups received once-daily intragastric administration for 60 days,while the con-trol group and model group received an equal volume of normal saline.Lung histopathology and collagen deposition were observed.Se-rum levels of inflammatory factors(TNF-α,IL-iβ and IL-6)and oxidative stress markers(MDA and SOD)were measured.Expression of MAPK and PI3K/Akt pathway-related proteins was detected by Western blot.Results:A total of 125 intersecting targets were identi-fied between SBT blood components and COPD,with key enrichment pathways including MAPK and PI3K-Akt.Animal experiments showed that compared with the model group,each SBT dose group reduced TNF-α、1L-1β、1L-6 levels,increased SOD activity,de-creased MDA content,and downregulated the protein expression of p-ERK,p-PI3K,p-AKT,p-p38,and p-JNK in lung tis-sue.Conclusion:SBT granules exert anti-inflammatory and anti-oxidative stress effects by simultaneously regulating the PI3K/AKT and MAPK signaling pathways,representing a key mecha-nism in its prevention of COPD.关键词
索罗西汤/网络药理学/分子对接/COPD/PI3K-AKT/MAPKKey words
Srolo Bzhtang/network pharmacology/mo-lecular docking/COPD/PI3K/AKT/MAPK分类
医药卫生引用本文复制引用
张琳枝,旦增央金,色珍,边巴顿珠,贡布,边巴次仁,叶本贵..基于网络药理学、分子对接和实验验证索罗西汤颗粒预防慢性阻塞性肺疾病的作用及机制研究[J].中药与临床,2026,17(4):35-43,中插10-中插11,11.基金项目
2023年中央对地方转移支付资金(中医药事业传承与发展)重点支持项目子项目中药创新能力提升项目 （中医药事业传承与发展）
2023年西藏自治区科技计划"揭榜挂帅"项目(XZ2023ZY0007G) （XZ2023ZY0007G）
