安徽农业大学学报2026，Vol.53Issue(3)：424-432,9.DOI:10.13610/j.cnki.1672-352x.20260713.007
'巴梨'炭疽病病原菌的分子鉴定及其生防菌筛选
Molecular identification and biocontrol agent screening for anthracnose pathogens in'Bartlett'pears
摘要
Abstract
[Objective]Pear anthracnose,caused by Colletotrichum species,severely affects pear leaves and fruits,leading to significant economic losses.To effectively control this disease,this study aimed to identify the pathogenic spe-cies responsible for anthracnose in European pears and screen for effective biocontrol yeasts.[Method]Three Colleto-trichum isolates(Bali-1-Bali-3)were purified and identified through pathogen isolation and purification,multilocus phy-logenetic analysis,and pathogenicity tests.[Result](1)Bali-1 was identified as Colletotrichum siamense,while Bali-2 and Bali-3 were identified as Colletotrichum fructicola.(2)Two biocontrol yeast strains,Hanseniaspora uvarum(Huv1)and Metschnikowia pulcherrima(Mp1),were selected and both showed significant inhibitory effects against the three patho-genic strains.(3)Plate inhibition assay results indicated that both Huv1 and Mp1 exhibited good inhibitory effects against the three pathogens.Specifically,the inhibition rates of Huv1 were 38.9%,40.7%,and 44.2%,respectively,while those of Mp1 were 34.6%,8.7%,and 27.7%,respectively.The fruit efficacy test showed that Huv1 and Mp1 inhibited the growth of the pathogens Bali-1 and Bali-2 to varying degrees.[Conclusion]This study clarifies that the dominant pathogens caus-ing anthracnose in'Bartlett'pears are C.siamense and C.fructicola,and identifies two promising biocontrol yeast strains,Huv1 and Mp1,providing a theoretical basis and microbial resources for the biological control of pear anthracnose.关键词
'巴梨'/炭疽病/病菌鉴定/多基因系统发育分析/生防菌筛选Key words
'Bartlett'pear/anthracnose/pathogen identification/multi-locus phylogenetic analysis/biocon-trol agent screening分类
农业科技引用本文复制引用
宋伟,汤小美,张慧慧,郭雨晴,曾家毅,李科霖,吴中营,郭献平,王东升,贾兵..'巴梨'炭疽病病原菌的分子鉴定及其生防菌筛选[J].安徽农业大学学报,2026,53(3):424-432,9.基金项目
国家自然科学基金项目(32302484) （32302484）
国家现代农业(梨)产业技术体系项目(CARS-28-14) （梨）
