草业学报2026，Vol.35Issue(8)：45-54,10.DOI:10.11686/cyxb2025330
喷施纳米氧化铈对紫花苜蓿幼苗耐盐性的影响
Effect of CeO2 NPs on the salt tolerance of alfalfa seedlings
摘要
Abstract
The aim of this work was to determine whether treatment with cerium dioxide nanoparticles(CeO2 NPs)increases the salt tolerance of alfalfa(Medicago sativa).A 50 mg·L-1 solution of CeO2 NPs was applied by foliar spraying to'Zhongmu No.1'alfalfa seedlings,and the seedlings were then subjected to a 10-day salt stress treatment with 300 mmol·L-1 NaCl.A range of parameters,including growth,photosynthetic characteristics,membrane lipid peroxidation,antioxidant enzyme activities,and osmotic regulatory substance contents were measured to comprehensively evaluate the effect of CeO2 NPs on the salt tolerance of alfalfa plants.Salt stress significantly inhibited seedling growth,reduced biomass,and photosynthetic capacity,and increased reactive oxygen species accumulation.When plants in the control group and those sprayed with CeO2 NPs were subjected to the salt treatment,those in the treatment group showed significantly milder symptoms of salt damage,increased seedling growth and biomass accumulation,and higher relative water content and photosynthetic performance.Under salt stress,the plants sprayed with CeO2 NPs exhibited significantly reduced relative conductivity,malondialdehyde content,and hydrogen peroxide and superoxide anion accumulation,and elevated activities of superoxide dismutase(SOD),ascorbate peroxidase(APX),catalase(CAT),and peroxidase(POD),as well as increased soluble sugar and proline contents.Specifically,in the CeO2 NPs-treated plants compared with control plants,the SOD,APX,CAT,and POD activities were increased by 20.0％,29.9％,41.4％,and 54.3％,respectively,while the soluble sugar and proline contents were increased by 39.4％and 16.5％,respectively,under salt stress.Collectively,these results show that CeO2 NPs promote photosynthesis and growth by enhancing antioxidant enzyme activities,promoting the accumulation of osmoprotectants,and decreasing oxidative damage,thereby enhancing alfalfa's salt tolerance.关键词
二氧化铈纳米颗粒/盐胁迫/紫花苜蓿/光合变化/生理响应Key words
cerium dioxide nanoparticles/salt stress/alfalfa/photosynthetic changes/physiological response引用本文复制引用
杨金辉,王晓彤,马永龙,余冬雯,童玉花,李淑霞..喷施纳米氧化铈对紫花苜蓿幼苗耐盐性的影响[J].草业学报,2026,35(8):45-54,10.基金项目
国家自然科学基金(U24A20426)和宁夏自然科学基金(2023AAC05019)资助. （U24A20426）
