草业学报2026，Vol.35Issue(8)：134-143,10.DOI:10.11686/cyxb2025334
25-羟基维生素D3对奶公牛生产性能和肌肉蛋白质合成基因表达的影响
Effects of 25-hydroxyvitamin D3 on growth performance and expression of genes related to protein synthesis in muscle of dairy bulls
摘要
Abstract
We evaluated the effects of 25-hydroxyvitamin D₃(CAL)and coated CAL(CCAL)on the average daily weight gain,nutrient digestibility,rumen fermentation,blood metabolites,and transcript profiles of genes related to protein synthesis in muscle of dairy bulls.Fifty Holstein bulls with(175.6±16.81)kg body weight and(6.0±0.2)months of age were assigned in a randomized block design to five groups with 10 bulls per group.Bulls in the control group received no supplementation,and those in the low CAL(LCAL),high CAL(HCAL),low CCAL(LCCAL),and high CCAL(HCCAL)groups received supplementation of 0.5 or 1.0 mg·d⁻1 in the form of CAL or CCAL,respectively.The experimental period was 70 days,with 10 days for adaptation.During the data and sample collection period,the body weights of bulls were measured,and samples of feed,feces,ruminal fluid,blood,and muscle were collected.1)It was found that with increasing CAL or CCAL supplementation,the average daily gain(ADG)increased linearly(P<0.05),and the feed-to-gain ratio decreased linearly(P<0.05).The apparent digestibility of dry matter,organic matter,and neutral detergent fiber increased linearly(P<0.05)with increasing CAL or CCAL supplementation.Ruminal total volatile fatty acid(TVFA)content and molar proportions of isobutyrate and isovalerate increased linearly(P<0.05),but the acetate-to-propionate ratio decreased linearly(P<0.05)with increasing CAL or CCAL supplementation.Blood total protein,albumin,insulin-like growth factor-1(IGF-1),total antioxidant capacity(T-AOC),glutathione peroxidase(GSH-Px),CAL,and 1,25-dihydroxyvitamin D₃ increased linearly(P<0.05),but the malondialdehyde content decreased linearly(P<0.05)with increasing CAL or CCAL supplementation.The transcript levels of IGF-1,IGF-1R,PI3K,mTOR,and P70S6K(encoding insulin-like growth factor-1,insulin-like growth factor-1 receptor,phosphatidylinositol 3-kinase,mechanistic target of rapamycin,and ribosomal protein S6 kinase,respectively)in muscle increased linearly(P<0.05)with increasing CAL or CCAL supplementation.2)Compared with bulls in the CAL groups,those in the CCAL groups had higher ADG,blood T-AOC,GSH-Px,CAL,and 1,25-dihydroxyvitamin D₃ levels and higher transcript levels of IGF-1,IGF-1R,PI3K,mTOR and P70S6K in muscle,similar nutrient digestibility and ruminal TVFA contents,but lower feed-to-gain ratio.These results indicate that CCAL is more effective than CAL for increasing the growth performance of bulls and decreasing the feed-to-gain ratio.关键词
25-羟基维生素D3/养分消化/瘤胃发酵/抗氧化状态/肌肉蛋白质合成/荷斯坦公牛Key words
25-hydroxyvitamin D₃/nutrient digestibility/rumen fermentation/antioxidant status/muscle protein synthesis/Holstein bull引用本文复制引用
张浩东,王聪,荆玉倩,冯家欣,刘百峤,冯江,孙楠,霍文婕,郭刚,刘强..25-羟基维生素D3对奶公牛生产性能和肌肉蛋白质合成基因表达的影响[J].草业学报,2026,35(8):134-143,10.基金项目
山西省肉牛产业技术创新战略联盟(2025CXYRH-048)和山西省现代农业牛产业技术体系建设项目(2025CYJSTX13)资助. （2025CXYRH-048）
