草业学报2026，Vol.35Issue(8)：157-169,13.DOI:10.11686/cyxb2025359
MfERF053增强活性氧清除能力和抗逆相关基因表达提高拟南芥抗旱性
MfERF053 enhances drought resistance of Arabidopsis thaliana by improving reactive oxygen species scavenging capacity and promoting expression of stress-resistance-related genes
摘要
Abstract
Drought stress restricts plant growth and agricultural production,and mining drought-tolerannce genes and analyzing their mechanisms are crucial for molecular breeding.Medicago falcata Mf ERF053 belongs to the AP2/ERF transcription factor family.Previous studies have shown that its expression is upregulated under drought stress,and Arabidopsis thaliana overexpressing this gene exhibits a drought-tolerant phenotype.However,the underlying mechanism remains unclear.In this study,A.thaliana Col-0 wild type(Col)and MfERF053-overexpressing lines(#19,#20,#33)were used as materials.After natural drought stress treatment,plant phenotypic,physiological,and molecular indicators were determined to clarify the drought-tolerance mechanism of MfERF053.The results showed that under drought stress:the overexpressing lines exhibited delayed leaf wilting and better recovery ability after rehydration;their relative water content(RWC)was significantly higher than that of Col(P<0.05),while their relative electrical conductivity(REC)and malondialdehyde(MDA)content were significantly lower than those of Col(P<0.05);the function of photosystem Ⅱ(PSⅡ)was more stable,with the maximum photochemical efficiency(Fv/Fm)and electron transport rate(ETR)significantly higher than those of Col(P<0.05);the degree of nitroblue tetrazolium(NBT)[for superoxide anion(·O₂⁻)localization]and 3,3'-diaminobenzidine(DAB)[for hydrogen peroxide(H₂O₂)localization]staining in leaves of the overexpressing lines was significantly lighter than that in Col,indicating reduced accumulation of·O₂⁻ and H₂O₂,and the activities of catalase(CAT)and peroxidase(POD)were significantly higher than those in Col(P<0.05).At the molecular level,under drought stress,the expression levels of stress-tolerance-related genes in MfERF053-overexpressing lines,including reactive oxygen species(ROS)-scavenging-related genes(AtCAT1,AtPOD3,AtSOD4),drought-responsive genes(AtRD22,AtRD29A),cold/drought cross-responsive genes(AtCOR15A,AtCOR47),potassium ion transport gene(AtHAK5),and abscisic acid(ABA)signaling pathway gene(AtABI5),were all significantly up-regulated(P<0.05).Most of these genes exhibited significantly higher expression levels in overexpressing lines than in Col(P<0.05),except that AtRD29A and AtABI5 in individual overexpressing lines showed no significant difference compared with Col.In conclusion,MfERF053 was found to enhance ROS scavenging capacity,upregulate the expression of stress-tolerant-related genes,and synergistically improve the physiological homeostasis and molecular response of A.thaliana under drought,ultimately improving its drought tolerance.This study provides important genetic resources and a theoretical basis for the molecular breeding of drought-tolerant crops/forages.关键词
MfERF053/拟南芥/抗旱性/活性氧清除/抗逆相关基因Key words
MfERF053/Arabidopsis thaliana/drought resistance/reactive oxygen species scavenging/stress-resistant-related genes引用本文复制引用
李倩,段伟,张雪莉,刘丽君,王玉祥..MfERF053增强活性氧清除能力和抗逆相关基因表达提高拟南芥抗旱性[J].草业学报,2026,35(8):157-169,13.基金项目
自治区高校基本科研业务费科研项目(XJEDU2023P054)资助. （XJEDU2023P054）
