草业学报2026，Vol.35Issue(8)：170-181,12.DOI:10.11686/cyxb2025088
藜麦NAC转录因子CqNAC66响应干旱和盐胁迫的功能研究
Functional analysis of quinoa NAC transcription factor CqNAC66 in response to drought and salt stress
摘要
Abstract
This research explored the function of quinoa(Chenopodium quinoa)NAC family member,CqNAC66 involved in drought resistance and salt tolerance.CqNAC66 was subcellularly localized in this study,and transgenic lines of Nicotiana benthamiana overexpressing CqNAC66 were obtained to compare the morphological and physiological responses of wild-type(WT)and transgenic plants after exposure to drought(20％PEG-6000)and salt(200 mmol·L-1 NaCl)stress.The results showed that CqNAC66 protein was subcellularly localized in the nucleus.Overexpression of CqNAC66 significantly decreased the water loss rates of excised leaves of tobacco plants.Under drought and salt stress treatment,overexpression of CqNAC66 in tobacco plants significantly decreased the leaf wilting index,and significantly increased the leaf surface area and aboveground fresh weight.In addition,overexpression of CqNAC66 significantly increased the relative content of chlorophyll(SPAD)and the activities of peroxidase,and significantly decreased the content of malondialdehyde in leaves of the studied tobacco plants.In conclusion,the heterologous expression of CqNAC66 in N.benthamiana improved the drought and salt tolerance of plants and CqNAC66 appeared to improve the resistance of transgenic tobacco by maintaining the stability of chlorophyll,regulating the plant antioxidant enzyme system and alleviating the cellular oxidative damage caused by stress.关键词
藜麦/CqNAC66/抗旱/耐盐/功能验证Key words
quinoa/CqNAC66/drought resistance/salt tolerance/functional verification引用本文复制引用
李凤,郭绪虎,曹慧芬,孔祥睿,杨冬雪,张东旭,张永芳..藜麦NAC转录因子CqNAC66响应干旱和盐胁迫的功能研究[J].草业学报,2026,35(8):170-181,12.基金项目
山西省基础研究计划(自由探索类)面上项目(202303021221167)和山西大同大学博士科研启动基金项目(2017-B-18)资助. （自由探索类）
