福建林业科技2026，Vol.53Issue(2)：58-65,8.DOI:10.13428/j.cnki.fjlk.2026.02.007
马尾松对松材线虫病抗性与耐受性差异的分子机制
Differential Molecular Mechanisms of Resistance and Tolerance to Pine Wilt Disease in Pinus massoniana
摘要
Abstract
To elucidate the molecular mechanisms underlying the differential responses of resistant and tolerant Pinus massoniana phenotypes to pine wilt disease(PWD),tolerant(TC),resistant(RC)and susceptible(NC)phenotypes were artificially inoculated with Bursaphelenchus xylophilus.Phenotypic differences among the phenotypes were compared,and transcriptome sequencing analysis was performed.The results showed that compared with NC,RC exhibited a significantly reduced mortality rate(P<0.01)and a signif-icantly lower pine wood nematode(PWN)load(P<0.01).TC also showed a significantly reduced mortality rate(P<0.01)but had a significantly higher PWN load(P<0.01).Transcriptome analysis revealed that,compared with NC,differentially expressed genes(DEGs)in TC were mainly enriched in pathways such as glutathione metabolism and diterpenoid biosynthesis.In contrast,DEGs in RC were primarily enriched in pathways related to cysteine and methionine metabolism and amino acid biosynthesis.These results suggest fundamental differences between TC and RC in metabolic regulation,energy metabolism,and defense-related metabolic path-ways.In conclusion,the resistance and tolerance of P.massoniana to PWD depend on distinct molecular mechanisms:resistant phe-notypes directly inhibit nematode proliferation through pathways such as fatty acid metabolism and terpenoid biosynthesis;tolerant phenotypes,on the other hand,mainly survive under high nematode load by strengthening antioxidant pathways such as glutathione metabolism to mitigate oxidative damage.This study provides a molecular basis for developing differential resistance breeding strate-gies for the prevention and control of pine wilt disease.关键词
马尾松/松材线虫/全长转录组/耐受性/抗性Key words
Pinus massoniana/Bursaphelenchus xylophilus/full-length transcriptome/tolerance/resistance分类
农业科技引用本文复制引用
张艳,王津津,管昊然,苏军,曾丽琼,詹芳芳,蔡守平..马尾松对松材线虫病抗性与耐受性差异的分子机制[J].福建林业科技,2026,53(2):58-65,8.基金项目
福建省林业科技项目(ZMGG-0807、ZMGG-0705) （ZMGG-0807、ZMGG-0705）
福建省科技计划项目(2026R1048) （2026R1048）
