分析化学2026，Vol.54Issue(7)：1133-1148,16.DOI:10.19756/j.issn.0253-3820.261139
基于微流控技术的单细胞转录组与蛋白质组整合分析研究进展
Microfluidics-based Integrated Analysis of Single-Cell Transcriptomics and Proteomics
摘要
Abstract
Single-cell multi-omics has become a crucial frontier in life sciences,providing powerful tools for investigating cellular heterogeneity and complex biological processes.Compared with single-omics analysis,the integration of single-cell transcriptomics and proteomics enables a more comprehensive characterization of the dynamic relationship between gene expression and cellular functional states,thereby offering multidimensional molecular information for understanding disease mechanisms.Benefiting from nanoliter-scale reaction systems and precise fluid manipulation,microfluidic technology has played an essential role in the development of integrated single-cell multi-omics analysis.In recent years,a series of microfluidics-based strategies have been developed for simultaneous analysis of single-cell transcriptomes and proteomes.According to the roles of microfluidic technologies in these approaches,the integration strategies can be categorized into three types:component separation strategies,barcode-assisted synchronous library construction strategies,and spatial labeling strategies.This review summarizes recent advances in microfluidics-based integrated single-cell transcriptomic and proteomic analysis from the perspectives of analytical principles,technical characteristics,as well as their advantages and limitations.Finally,the key technical challenges and future development trends in this field are discussed.关键词
微流控/单细胞分析/转录组学/蛋白质组学/评述Key words
Microfluidics/Single-cell analysis/Transcriptomics/Proteomics/Review引用本文复制引用
范玟慷,蒋易蓉,郑欣雨,潘建章,方群..基于微流控技术的单细胞转录组与蛋白质组整合分析研究进展[J].分析化学,2026,54(7):1133-1148,16.基金项目
国家自然科学基金重点项目(No.22234007)和国家重大科研仪器研制项目(No.22527805)资助. Supported by the Key Program of the National Natural Science Foundation of China(No.22234007)and the National Major Scientific Research Instrument Development Project(No.22527805). （No.22234007）
