分析化学2026，Vol.54Issue(7)：1174-1183,10.DOI:10.19756/j.issn.0253-3820.261023
熵驱动介导传感器在miRNA检测中的研究进展
Progresses in Entropy-driven Mediated Sensors for miRNA Detection
摘要
Abstract
MicroRNAs(miRNAs)are important non-coding RNA molecules,whose expression levels play a significant role in various physiological and pathological processes.The entropy-driven reaction(EDR)offers a novel method for amplifying miRNA signal into more stable DNA signal through a miRNA-initiated cyclic process,all steps under non-enzymatic catalysis and isothermal conditions.This approach not only facilitates the direct detection of DNA but also enables subsequent analyses utilizing the DNA products generated in the process.In contrast to conventional miRNA analysis techniques,EDR is operated without specialized reagents or equipment,solely harnessing entropy changes within the system to achieve necessary sensitivity and selectivity.Additionally,the EDR process is straightforward and flexible.Based on these characteristics,a range of innovative miRNA sensing strategies and detection methods have been developed utilizing EDR assay.This review summarized three major research directions in miRNA detection,including direct EDR-based detection,EDR combined with isothermal amplification,and EDR combined with nanomaterials,in which their typical applications and advantages were discussed.Finally,this review highlighted the current challenges and future development trends related to EDR-mediated miRNA biosensors,with the aim of providing a theoretical foundation for the development of more sensitive and accurate sensors in the future.关键词
微小核糖核酸/熵驱动反应/等温扩增技术/纳米材料/评述Key words
Micro ribonucleic acid/Entropy-driven reaction/Isothermal amplification technology/Nanomaterials/Review引用本文复制引用
杨丽,侯小曼,李汶鞠,曹汝菲,段晓雷..熵驱动介导传感器在miRNA检测中的研究进展[J].分析化学,2026,54(7):1174-1183,10.基金项目
国家自然科学基金项目(Nos.82160409,82560415)、贵州省科技合作基础研究项目(Nos.MS(2025)389,ZK[2023]523)和省级大学生创新创业项目(No.ZYDC202402195)资助. Supported by the National Natural Science Foundation of China(Nos.82160409,82560415),the Science&Technology Fun Program for Basic Research of Guizhou Province(Nos.MS(2025)389,ZK[2023]523)and the Provincial College Students'Innovation and Entrepreneurship Training Program(No.ZYDC202402195). （Nos.82160409,82560415）
