分析化学2026，Vol.54Issue(7)：1191-1199,9.DOI:10.19756/j.issn.0253-3820.261182
线形离子阱电子捕获解离装置的设计与实验研究
Design and Experimental Study of Linear-Ion-Trap Electron Capture Dissociation Device
摘要
Abstract
Electron capture dissociation(ECD)is an important dissociation method for characterizing post-translational modifications of biomolecules in protein mass spectrometry,serving as a complement to traditional tandem mass spectrometry.In this study,an ECD device based on digital linear ion trap(DLIT)technology was designed,and an electron emission source equipped with an elongated hot cathode was developed to provide low-energy electrons for ECD reactions.SIMION software was employed to simulate and optimize the electron transmission system,and the simulation results showed that the electron transmission efficiency of the device reached 90.66%.Cathode emission characterization tests were further performed to investigate the relationship between the cathode bias voltage and the electron beam current reaching the ion reaction region,and to determine the operating parameters of the emission source.Finally,in-trap ECD experiments were conducted using the standard peptide Substance P.The results demonstrated that the cathode generated sufficient reaction electrons,and adjusting the bias voltage effectively increased the number of electrons entering the ion trap.Successful experiments produced tandem mass spectra,enabling accurate peptide sequencing,thereby validating the feasibility of the developed device.关键词
电子捕获解离/数字线形离子阱/长条型热阴极/蛋白质质谱分析Key words
Electron capture dissociation/Digital linear ion trap/Elongated hot cathode/Protein mass spectrometry analysis引用本文复制引用
宋子洋,谢智超,倪福中,陈剑峰,周东东,徐福兴,丁力..线形离子阱电子捕获解离装置的设计与实验研究[J].分析化学,2026,54(7):1191-1199,9.基金项目
国家重点研发计划项目(Nos.2023YFF0721001,2024YFF0725800)和国家自然科学基金项目(No.22574088)资助. Supported by the National Key R&D Program of China(Nos.2023YFF0721001,2024YFF0725800)and the National Natural Science Foundation of China(No.2574088). （Nos.2023YFF0721001,2024YFF0725800）
