广州中医药大学学报2026，Vol.43Issue(8)：2202-2209,8.DOI:10.13359/j.cnki.gzxbtcm.2026.08.025
万氏清心化痰汤对脑小血管病大鼠神经血管单元及AMPK/SIRT1/PGC1α通路的影响
Wan's Qingxin Huatan Decoction Attenuates Cerebral Small Vessel Disease in Rats by Modulating the Neurovascular Unit and the AMPK/SIRT1/PGC1α Pathway
摘要
Abstract
Objective To investigate the therapeutic effect and mechanism of Wan's Qingxin Huatan Decoction in rats with cerebral small vessel disease(CSVD).Methods The CSVD model was established by a high-fat diet combined with a modified two-vessel occlusion(2-VO)method.Successfully modeled rats were randomly divided into a model group,low-,medium-,and high-dose Wan's Qingxin Huatan Decoction groups,and a nimodipine group,with a sham operation group also established(n=12 per group).After the respective interventions,cognitive function was assessed using the Morris water maze test(escape latency,number of platform crossings,and time spent in the target quadrant).Histopathological changes in the hippocampus were observed by hematoxylin-eosin(HE)staining.Nissl staining was used to evaluate the morphology,number,and distribution of Nissl bodies in hippocampal neurons.Golgi staining was performed to observe dendrites changes in hippocampal neurons.Western Blot analysis was conducted to detect the expression of neurovascular unit-related proteins[glial fibrillary acidic protein(GFAP),aquaporin-4(AQP-4),neuronal nuclei antigen(NeuN),and microtubule-associated protein 2(MAP-2)],as well as proteins involved in the AMP-activated protein kinase(AMPK)/sirtuin 1(SIRT1)/peroxisome proliferator-activated receptor gamma coactivator 1-alpha(PGC1α)pathway.Results Compared with the sham operation group,the model group exhibited sparsely arranged and irregularly shaped neurons in the hippocampus,a reduced number of Nissl bodies,prolonged escape latency,and increased relative protein expression of GFAP and AQP-4 in brain tissue(P＜0.05),while the number of platform crossings,time spent in the target quadrant,total dendritic length,dendritic spine density,and relative protein expression of NeuN,MAP-2,p-AMPK/AMPK,SIRT1,and PGC1α were significantly decreased(P＜0.05).Compared with the model group,the low-,medium-,and high-dose Wan's Qingxin Huatan Decoction groups and the nimodipine group showed more regularly arranged neurons,improved morphological structure,an increased number of Nissl bodies,shortened escape latency,and decreased relative protein expression of GFAP and AQP-4,along with increases in the number of platform crossings,time spent in the target quadrant,total dendritic length,dendritic spine density,and the relative protein expression of NeuN,MAP-2,p-AMPK/AMPK,SIRT1,and PGC1α(P＜0.05).No statistically significant differences were observed in any of these indicators between the high-dose Wan's Qingxin Huatan Decoction group and the nimodipine group(P＞0.05).Conclusion Wan's Qingxin Huatan Decoction can activate the AMPK/SIRT1/PGC1α pathway,repair the neurovascular unit,protect brain function and structure,and ameliorate cognitive impairment in rats with CSVD.关键词
万氏清心化痰汤/脑小血管病/神经血管单元/AMPK/SIRT1/PGC1α通路/大鼠Key words
Wan's Qingxin Huatan Decoction/cerebral small vessel disease(CSVD)/neurovascular unit/AMPK/SIRT1/PGC1α pathway/rats分类
医药卫生引用本文复制引用
刘麒麟,姜磊,靳晶,张雯,杨畅,赵文静,罗艺璇..万氏清心化痰汤对脑小血管病大鼠神经血管单元及AMPK/SIRT1/PGC1α通路的影响[J].广州中医药大学学报,2026,43(8):2202-2209,8.基金项目
湖北省时珍人才工程科研项目(鄂卫函[2024]256号) （鄂卫函[2024]256号）
武汉市中医药科研项目(编号:WZ24B42) （编号:WZ24B42）
