广州中医药大学学报2026，Vol.43Issue(8)：2243-2248,6.DOI:10.13359/j.cnki.gzxbtcm.2026.08.030
玉竹转录组测序及生物信息学分析
Transcriptome Sequencing and Bioinformatics Analysis of Polygonatum odoratum
摘要
Abstract
Objective To analyze the transcriptome expression characteristics of Polygonatum odoratum.Methods Total RNA was extracted from Polygonatum odoratum,and a cDNA library was constructed for high-throughput sequencing.Unigene sequences were obtained through de novo assembly.Genetic information at the transcriptome level was deciphered through sequence annotation and comparative analysis.Results A total of 76 335 unigenes were identified,with an average length of 807 bp and an N50 value of 1 245 bp.Among them,36 550 unigenes were functionally annotated in at least one database,and Polygonatum odoratum exhibited the highest sequence similarity to Asparagus officinalis.In the KEGG database,23 136 unigenes were annotated,including enzyme genes involved in terpenoid,flavonoid,and polysaccharide biosynthesis pathways,numbering 78,76,and 329,respectively.In the GO database,28 909 unigenes were annotated,covering categories such as cellular anatomical entity,binding,and cellular process.Furthermore,MISA analysis of 18 055 unigenes longer than 1 000 bp identified 7 623 simple sequence repeat(SSR)loci,and corresponding primers were designed.Additionally,by aligning the unigenes against the plantTFDB database,367 transcription factors including MYB,AP2/ERF,WRKY,and bHLH were identified.Conclusion The transcriptome data generated in this study provide a crucial foundation for in-depth exploration of the biosynthetic pathways and functional genes of medicinal components in Polygonatum odoratum,and also support its resource conservation and development.关键词
玉竹/转录组/非冗余基因/功能注释/简单序列重复Key words
Polygonatum odoratum/transcriptome/unigene/functional annotation/simple sequence repeat(SSR)分类
医药卫生引用本文复制引用
李静宇,顾艳,蔡时可,王继华..玉竹转录组测序及生物信息学分析[J].广州中医药大学学报,2026,43(8):2243-2248,6.基金项目
以农产品为单元的广东省现代农业产业技术体系创新团队建设项目(编号:2024CXTD24) （编号:2024CXTD24）
清远南药产业5个乡村小微企业技术服务项目(编号:BNGC2024-QY-0011) （编号:BNGC2024-QY-0011）
广东省农业科学院汕尾分院科技合作专项(编号:202302) （编号:202302）
