核农学报2026，Vol.40Issue(9)：1718-1728,11.DOI:10.11869/j.issn.1000-8551.2026.09.1718
甘薯PIN基因家族鉴定和非生物胁迫下的表达分析
Genome-Wide Identification and Expression Analysis of PIN Gene Family in Sweetpotato under Abiotic Stresses
摘要
Abstract
The auxin efflux transporter PIN is a key factor mediating auxin polar transport and maintaining auxin concentration gradients,playing a crucial role in plant growth,development,and abiotic stress responses.To investigate the biological functions of the PIN gene family in sweetpotato(Ipomoea batatas),this study employed an integrated bioinformatics and transcriptomics approach to identify its family members and analyze their response patterns to abiotic stresses in sweetpotato.The results showed that a total of 17 PIN family genes(IbPIN1-IbPIN17)were identified in the sweetpotato genome,which are unevenly distributed across 9 chromosomes.All their encoded proteins contain 1-4 conserved Mem_trans domains and are predominantly localized to the plasma membrane.Phylogenetic analysis classified this family into 6 subclades and revealed 9,22,and 21 collinear gene pairs between sweetpotato with Arabidopsis thaliana,Ipomoea triloba,and Ipomoea trifida,respectively.Promoter cis-element analysis indicated that these genes contain a total of 38 cis-acting elements associated with stress response,growth and development,hormone response,and light response.Transcriptome profiling demonstrated that IbPIN genes are actively involved in the response to abiotic stress.Among them,the expression of IbPIN2,IbPIN3,IbPIN4,IbPIN5,IbPIN7,and IbPIN16 was significantly upregulated under heat stress,whereas their expressions were markedly suppressed under drought or salt stress.These findings suggest that these six genes may play a key role in regulating the response of sweetpotato to adverse environmental conditions.This study provides a systematic reference for functional characterization of sweetpotato PIN genes and offers valuable candidate gene resources for breeding stress-resistant sweetpotato varieties.关键词
甘薯/PIN基因家族/生物信息学/非生物胁迫/基因表达Key words
sweetpotato/PIN gene family/bioinformatics/abiotic stress/gene expression分类
农业科技引用本文复制引用
高鹏,许正艳,管荻,郎涛,屈会娟,冯俊彦,蒲志刚,张聪..甘薯PIN基因家族鉴定和非生物胁迫下的表达分析[J].核农学报,2026,40(9):1718-1728,11.基金项目
四川省财政自主创新专项(2022ZZCX029),四川省农业科学院科研条件平台建设专项"2025生物技术育种工程中心运转费"(51000023Y000008258891),四川省农业科学院科技成果中试熟化与示范转化项目(2025ZSSFJD06) （2022ZZCX029）
