核农学报2026，Vol.40Issue(9)：1740-1752,13.DOI:10.11869/j.issn.1000-8551.2026.09.1740
烟草Tubby-like基因家族鉴定及非生物胁迫表达分析
Identification of Tubby-like Gene Family in Tobacco(Nicotiana tabacum)and Analysis of Abiotic Stress Expression
摘要
Abstract
The plant Tubby-like protein(TLP)gene family plays a crucial regulatory role in diverse biological processes,including abiotic stress responses and growth and development.To elucidate the molecular mechanisms underlying the response of tobacco TLP genes to abiotic stress,this study employed bioinformatics approaches to identify TLP family members in tobacco.A comprehensive analysis was conducted,encompassing phylogenetic tree construction,cis-acting element prediction,conserved domain and motif characterization,protein-protein interaction(PPI)network prediction,and expression profiling.The results demonstrated that a total of 26 NtTLP family genes were identified across the entire tobacco genome.The encoded proteins varied in length from 88 to 741 amino acids(aa),with molecular weights ranging from 10.14 to 83.27 kDa and isoelectric points(pI)spanning 4.72 to 9.78.Subcellular localization prediction revealed that the majority of NtTLP members(17 out of 26)were localized in the nucleus.Promoter analysis indicated that the cis-acting elements within the NtTLP genes could be categorized into three major classes:hormone-responsive elements,stress-responsive elements,and elements associated with growth,development and biological processes.Among these,hormone-responsive elements were the most abundant,with up to 14 distinct types.Furthermore,tissue expression profiling showed that NtTLP family genes exhibited tissue-and developmental stage-specific expression patterns in tobacco,with generally high expression levels detected in seeds.Protein-protein interaction prediction identified NtTLP02 and NtTLP06 as hub proteins,potentially engaging in extensive interaction networks with other NtTLP members.Quantitative real-time PCR(qRT-PCR)analysis indicated that both NtTLP02 and NtTLP06 responded to heat,salt,cold,and drought stresses to varying degrees;Notably,NtTLP02 displayed a more sensitive response to cold and drought stresses.Subsequent subcellular localization assays confirmed that both NtTLP02 and NtTLP06 proteins were localized in the nucleus.Collectively,these findings suggest that NtTLP02 and NtTLP06 are important candidate genes mediating tobacco responses to abiotic stresses.This study provides valuable genetic resources for further elucidating the regulatory mechanisms underlying abiotic stress tolerance in tobacco.关键词
烟草/Tubby-like蛋白/非生物胁迫/表达分析Key words
tobacco/Tubby-like protein/abiotic stress/expression analysis分类
农业科技引用本文复制引用
李辉,林文梁,杨毅..烟草Tubby-like基因家族鉴定及非生物胁迫表达分析[J].核农学报,2026,40(9):1740-1752,13.基金项目
河北中烟科技一般项目"烟叶理化指标与感官的相关性体系构建及烟叶质量特征的评价应用"(HBZY2025A021) （HBZY2025A021）
