核农学报2026，Vol.40Issue(9)：1761-1768,8.DOI:10.11869/j.issn.1000-8551.2026.09.1761
基于代谢组学和转录组学的韦氏曲霉侵染葡萄研究
Metabolomic and Transcriptomic Analysis of Aspergillus westerdijkiae during Grape Infection
摘要
Abstract
To investigate the metabolic characteristics and potential biomarkers of Aspergillus westerdijkiae after infecting grape berries.Summer Black grapes were inoculated with strain fc-1 and incubated at 28℃for 7 d.Non-targeted metabolomic analysis were performed using high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(HPLC-QTOF-MS/MS),and Pearson correlation analysis was conducted between differential metabolites and differentially expressed genes identified from transcriptomic data.A.westerdijkiae successfully colonized the grape surface and induced visible lesions,showed great differences from those grown on potato dextrose agar.A total of 146 differential metabolites were identified,mainly including lipids,organic acids and sugar alcohols.These metabolites were enriched in the lysine degradation,galactose metabolism and fatty acid biosynthesis pathways.Inositol and dulcitol were significantly up-regulated and positively associated with TU_AoFC_00318(encoding the urea amidolyase α-subunit),TU_AoFC_02007(encoding methionine transporter),TU_AoFC_09517(encoding endo-1,6-α-mannanase),and TU_AoFC_10219(encoding polyketide synthase),while showing significant negative correlations with TU_AoFC_00835/09062(encoding copper transporters),TU_AoFC_01162(encoding dehydrogenase),and TU_AoFC_08458(encoding transporter).The results of this study provide a scientific basis for elucidating the pathogenic mechanism of A.westerdijkiae and for establishing a metabolism-based strategy for the prevention,control,and early warning of grape diseases.关键词
韦氏曲霉/葡萄/转录组/代谢组Key words
Aspergillus westerdijkiae/grapes/transcriptomics/metabolomics分类
农业科技引用本文复制引用
胡亚凡,王刚,刘一冰,李放放,邢福国..基于代谢组学和转录组学的韦氏曲霉侵染葡萄研究[J].核农学报,2026,40(9):1761-1768,8.基金项目
国家重点研发计划课题(2022YFD2100104) （2022YFD2100104）
