海洋科学2026，Vol.50Issue(2)：109-118,10.DOI:10.11759/hykx20260119002
星突江鲽精子诱导圆斑星鲽雌核发育和异源三倍体的研究
Induction of gynogenesis and hybrid triploidy in spotted hal-ibut(Verasper variegatus)using starry flounder(Platichthys stellatus)sperm
摘要
Abstract
Female spotted halibut(Verasper variegatus)exhibit a substantial growth advantage,which can be fully exploited via sex-controlled breeding to produce all-female populations.Meanwhile,polyploid breeding can gener-ate sterile groups,potentially enhancing growth rates.Both approaches represent important strategies for improving aquaculture efficiency.Therefore,to establish an efficient breeding technology system,sperm from the starry flounder(Platichthys stellatus)was used as an activating source in this study.By optimizing key parameters,in-cluding ultraviolet(UV)irradiation for genetic inactivation and cold shock to suppress the release of the second polar body,gynogenetic diploids and hybrid triploids of spotted halibut were successfully induced.The develop-mental characteristics of embryos with different ploidy levels were compared.The results showed high hybridiza-tion affinity between P.stellatus sperm and V.variegatus eggs.Under a sperm suspension depth of 0.4 mm,the op-timal UV dose for complete sperm genetic inactivation was 72.0 mJ·cm-2.Furthermore,the optimal cold-shock conditions for inducing hybrid triploids were a treatment initiated at 7 min post-fertilization for 35-45 min at-1.8℃,under which the triploidy rate approached 100%.Comparative embryological analysis revealed considera-ble differences among the ploidy groups in developmental timing,morphology,and hatching performance:haploids displayed typical haploid syndrome;hybrid diploids developed fastest but had lower hatching rates and developed yellowish-green pigmentation on the tail fin fold;gynogenetic diploids exhibited embryonic morphology consistent with that of the control group but demonstrated the lowest hatching rate and highest deformity rate(excluding hap-loids);hybrid triploids hatched earlier than normal diploid controls and shared the same yellowish-green pigmenta-tion as hybrid diploids,but the newly hatched larvae presented a higher deformity rate.In summary,this study es-tablishes a stable technical system for inducing gynogenetic diploids and hybrid triploids in spotted halibut using P.stellatus sperm,thereby providing reliable technical support and a theoretical basis for sex-control breeding,poly-ploid breeding,and germplasm innovation in this species.关键词
圆斑星鲽/星突江鲽/雌核发育/杂交三倍体/胚胎发育Key words
Verasper variegatus/Platichthys stellatus/gynogenesis/hybrid triploid/embryonic development分类
农业科技引用本文复制引用
范鑫,孙晓璇,徐荣静,成路遥,秦搏,徐文腾,孟振..星突江鲽精子诱导圆斑星鲽雌核发育和异源三倍体的研究[J].海洋科学,2026,50(2):109-118,10.基金项目
山东省重点研发计划(农业良种工程)项目(2025LZGC039) （农业良种工程）
中国水产科学研究院基本科研业务费(2025XT0502) （2025XT0502）
国家重点研发计划课题(2022YFD2400402) （2022YFD2400402）
国家现代农业产业技术体系(CARS-047-G31)[the Agricultural Variety Improvement Project of Shandong Province,No.2025LZGC039 （CARS-047-G31）
Central Public-interest Scientific Institution Basal Research Fund,CAFS,No.2025XT0502 （）
the National Key R&D Program of China,No.2022YFD2400402 （）
the China Agriculture Research System,No.CARS-047-G31] （）
