经济动物学报2026，Vol.30Issue(4)：280-288,9.DOI:10.13326/j.jea.2026.1950
表达共轭亚油酸异构酶mcra基因与菌毛亚基工程益生菌的构建及特性
Construction and Characteristics of Engineered Probiotics Incorporating the mcra Gene for Conjugated Linoleic Acid Isomerase and Fimbria Subunit Engineering
摘要
Abstract
This study aimed to develop engineered probiotics based on Lactobacillus and Escherichia coli and evaluate their adhesion and invasion abilities to intestinal epithelial cells,as well as their competitive inhibition against enterotoxigenic Escherichia coli K88.The mcra gene encoding the linoleate isomerase from Lactobacillus rhamnosus GG and the genes encoding the bifidobacterial adhesive lipoprotein BOPA along with the Enterotoxigenic E.coli K88 fimbrial proteins FaeG and FaeC were successfully expressed by the delivery vectors of Lactobacillus casei ATCC 393 and Escherichia coli Nissle 1917(EcN),respectively.The engineered probiotics were designated as LC-pPG-mcra and EcN-pPG-BOPA/pBbB8K-FaeC-FaeG(EcN-BFF).The adhesion and invasive capabilities of two engineered probiotic strains to Caco-2 intestinal epithelial cells,as well as their competitive adhesion and competitive invasion against ETEC K88,were examined.The results demonstrated that both engineered strains exhibited high adhesion and invasive capabilities to Caco-2 cells.Furthermore,they significantly inhibited the adhesion and subsequent invasion to Caco-2 cells by the pathogenic K88 strain through competitive mechanisms.In conclusion,two engineered probiotics with favorable adhesion,invasion,and anti-infective properties were successfully constructed,these results lays the foundation for the application of microecological agents in the prevention and treatment of bacterial intestinal diseases.关键词
产肠毒素大肠杆菌/mcra基因/菌毛亚基/工程益生菌Key words
enterotoxigenic Escherichia coli/mcra gene/fimbria subunit/engineered probiotic分类
农业科技引用本文复制引用
李惠,张婷婷,邵鑫,马冬妮,林震堃,白帆,王娟,张冬星..表达共轭亚油酸异构酶mcra基因与菌毛亚基工程益生菌的构建及特性[J].经济动物学报,2026,30(4):280-288,9.基金项目
国家自然科学基金青年科学基金项目(32102681) （32102681）
