集美大学学报（自然科学版）2026，Vol.31Issue(4)：379-397,19.DOI:10.19715/j.jmuzr.2026.04.01
GFP标记的alt基因缺失嗜水气单胞菌株的构建
Construction of GFP-Labeled Aeromonas hydrophila Strains with alt Gene Deletion
摘要
Abstract
To elucidate the biological functions and pathogenic mechanisms of the heat-labile cytotonic en-terotoxin/lipase gene(alt/lipase)in Aeromonas hydrophila in the process of infection,this study used A.hy-drophila strain B11 isolated from eels as the research material.By employing fluorescence labeling and gene knockout technologies,four engineered strains GFP-labeled strain B11::GFP,alt/lipase gene knockout strain B11::GFP Δalt/lipase,lipase complementation strainB11::GFP C-lipase and alt complementation strain B11::GFP C-alt were successfully constructed.Characterization of these strains revealed that deletion of alt/lipase didn't affect their growth kinetics,but significantly impaired the biofilm formation capacity.Furthermore,laser confocal fluorescence microscopy demonstrated the infection locations of these strains in zebrafish.关键词
嗜水气单胞菌/绿色荧光蛋白标记/不耐热肠毒素/脂肪酶/基因缺失株Key words
Aeromonas hydrophila/green fluorescent protein(GFP)-labeled/heat-labile cytotonic entero-toxin/lipase/gene-deleted mutant分类
农业科技引用本文复制引用
吴雨薇,黄文树,李东利,黄贝,梁英,徐继松,熊静..GFP标记的alt基因缺失嗜水气单胞菌株的构建[J].集美大学学报（自然科学版）,2026,31(4):379-397,19.基金项目
国家重点研发计划重点专项(2024YFD2401003) （2024YFD2401003）
厦门市自然科学基金项目(3502Z20227206) （3502Z20227206）
