集美大学学报（自然科学版）2026，Vol.31Issue(4)：398-407,10.DOI:10.19715/j.jmuzr.2026.04.02
大黄鱼锥体虫PCR检测方法的建立与应用
Development and Application of PCR Detection Method for Trypanosoma in Larimichthys crocea
摘要
Abstract
To achieve effective prevention and control of the trypanosomosis and reduce economic losses,two pairs of specific primers were designed,and a PCR detection method based on the conserved sequence of the trypanosome 18S rRNA gene in Larimichthys crocea was established.The accuracy and sensitivity of the method were systematically evaluated through primer specificity verification,sensitivity testing,and practical sample detection.The results showed that this method produced specific amplification only for trypanosome DNA from L.crocea,with no cross-reactivity observed for non-target pathogens such as scuticociliate,Pseudo-monas putida,iridovirus,or DNA from healthy L.crocea tissues,demonstrating good specificity.The minimum detection limits of F1/R1 and F2/R2 for trypanosome were 6.52×10-39 and 6.85×10-37 ng/L,respectively,indicating high detection sensitivity.Among the 10 L.crocea samples tested,this method detected 9 positive samples,significantly outperforming the microscopy method(which detected 7 positives),further confirming its accuracy and sensitivity in practical sample detection.关键词
大黄鱼/锥体虫/PCR检测/早期诊断Key words
Larimichthys crocea/Trypanosoma/PCR test/early diagnosis分类
农业科技引用本文复制引用
程志强,陈燕婷,张东玲..大黄鱼锥体虫PCR检测方法的建立与应用[J].集美大学学报（自然科学版）,2026,31(4):398-407,10.基金项目
福建省促进海洋与渔业产业高质量发展专项资金项目(FJHYF-L-2025-02-012) （FJHYF-L-2025-02-012）
大黄鱼种业创新与产业化开发企业联合横向项目(2021FJSC2Y01) （2021FJSC2Y01）
