集美大学学报（自然科学版）2026，Vol.31Issue(4)：423-434,12.DOI:10.19715/j.jmuzr.2026.04.04
3种柑橘类精油对食源性致病菌的抑菌活性及协同增效作用
The Antibacterial Effects and Synergistic Interactions of Three Citrus Essential Oils Against Common Foodborne Pathogenic Bacteria
王鑫 1张晨 2俞媛瑞 3亢凯杰 3单露英3
作者信息
- 1. 四川轻化工大学食品与酿酒工程学院,四川 宜宾 644002
- 2. 重庆第二师范学院生物与化学工程学院,重庆 400067
- 3. 昭通学院化学化工学院,云南 昭通 657000
- 折叠
摘要
Abstract
Using Escherichia coli,Shigella spp.and Staphylococcus aureus as indicator bacteria,the anti-bacterial activities of three citrus essential oils were evaluated and the combinations with synergistic effects were screened.The Kirby-Bauer disc diffusion assay showed that bergamot,yellow lemon,and green lemon es-sential oils formed inhibition zones(10.09-26.38 mm),with minimum inhibitory concentration(MIC)of 3.75-15 mL/L and minimum bactericidal concentration(MBC)of 7.5-60 mL/L,respectively.Bergamot essential oil exhibited antibacterial activity against all three tested bacterial strains with the lowest MIC values(3.75 mL/L for each),indicating the strongest and broad-spectrum antibacterial effect.Checkerboard dilution tests revealed a synergistic effect(FICI=0.5)at a volume ratio of 1∶1(V/V)yellow lemon and bergamot essential oil combination against E.coli,reducing the MIC from 3.75 mL/L to 0.9375 mL/L.While,it exhibited FICI values of 0.75 against S.aureus and 1.25 against Shigella spp.,indicating additive and indifferent effect respectively.In a chilled chicken breast model at 0-4℃,the total bacterial count on day 7 of the combined es-sential oil-activated carbon sustained-release treatment was 3.955 lg CFU/g,which was significantly lower than that of the control group(4.956 lg CFU/g,P<0.05),and extended the period during which the total viable count remained≤5 lg CFU/g from 7 to 10 days.关键词
食源性致病菌/柑橘类精油/最小抑菌浓度/最小杀菌浓度/分级抑菌浓度指数Key words
foodborne pathogens/citrus essential oils/minimum inhibitory concentration/minimum bacteri-cidal concentration/fractional inhibitory concentration index分类
轻工纺织引用本文复制引用
王鑫,张晨,俞媛瑞,亢凯杰,单露英..3种柑橘类精油对食源性致病菌的抑菌活性及协同增效作用[J].集美大学学报（自然科学版）,2026,31(4):423-434,12.
