江苏农业学报2026,Vol.42Issue(7):1317-1325,9.DOI:10.3969/j.issn.1000-4440.2026.07.003
普通小麦TaSeBP基因表达与自然等位变异分析
Expression and natural allelic variation analysis of TaSeBP gene in com-mon wheat
摘要
Abstract
This study applied bioinformatics and molecular biology methods to analyze the expression and natural al-lelic variation of the wheat SeBP gene,providing theoretical basis and methods for screening and developing selenium-rich wheat germplasm resources.Through the analysis of expression levels of SeBP gene in different wheat varieties and studies under stress treatments,the results showed that TaSeBP-3D had the highest expression level.Furthermore,TaSeBP respon-ded to selenium supplementation and cold stress,with Huaimai 18 showing an upregulation in relative expression,while Jinghe No.1 showed a decrease in relative expression.Analysis of cis-acting elements in the candidate promoter region re-vealed the presence of abscisic acid-responsive,gibberellin-responsive elements,suggesting a relationship with plant growth and development and response to stress.DNA variation analysis in natural wheat populations indi-cated that TaSeBP had multiple missense mutation sites and single nucleotide insertion-deletion variations.Phylo-genetic and evolutionary analysis of wheat SeBP revealed differentiation between monocotyledonous and dicotyledon-ous plants.The results of this study can provide a reference for in-depth research on TaSeBP gene function and its applica-tion in molecular breeding.关键词
普通小麦/硒结合蛋白/硒添加/寒冷胁迫/自然等位变异Key words
common wheat(Triticum aestivum L.)/selenium-binding protein/selenium supplement/freezing stress/natural allelic variation分类
农业科技引用本文复制引用
王新宇,吕晋慧,姜亮,张春来,刘卓君,郭子浩,史小霞,郝昱宁,韦小龙,杨博慧,贾举庆,杨珍平..普通小麦TaSeBP基因表达与自然等位变异分析[J].江苏农业学报,2026,42(7):1317-1325,9.基金项目
国家自然科学基金项目(31971944) (31971944)
山西省教育厅研究生教育创新计划实践创新项目(2023SJ109) (2023SJ109)
山西省教育厅专业建设项目(J20220211) (J20220211)
山西农谷建设科研专项(SXNGJSKYZX201702) (SXNGJSKYZX201702)