江苏农业学报2026，Vol.42Issue(7)：1326-1337,12.DOI:10.3969/j.issn.1000-4440.2026.07.004
小麦TaXI-III互作蛋白的筛选及其与TaHRLI-2B的互作分析
Screening for TaXI-III interacting proteins in wheat and analysis of its in-teraction with TaHRLI-2B
摘要
Abstract
In order to further explore the molecular mechanism of the wheat xylanase inhibitor gene TaXI-III in re-sistance to Fusarium graminearum,this study cloned the TaXI-III gene from the moderately resistant variety Annong 1589.Bioinformatics and subcellular localization analysis revealed that the TaXI-III protein was localized to the cell membrane/wall.The bait vector was constructed by homologous recombination method.The self-activation and functional assays con-firmed that pBT3N-TaXI-III had no self-activation activity in NMY51 yeast cells,and could produce functional proteins.A total of 19 candidate proteins that may interact with TaXI-III were finally obtained by split-ubiquitin yeast two-hybrid system from wheat cDNA library.These proteins were associated with diverse biological functions,including signal transduction,transmembrane transport,programmed cell death,and stress response.Results from yeast retransformation,luciferase complementation assay and bimolecular fluores-cence complementation assay confirmed that TaHRLI-2B,an HR-like lesion-inducing protein involved in the hyper-sensitive response,interacted with TaXI-III in both yeast and plant cells.The expression of TaHRLI-2B was induced by Fusarium graminearum infection through quantitative real-time reverse transcription polymerase chain reaction(RT-qPCR)analysis.And the overexpression of TaHRLI-2B in Arabidopsis thaliana enhanced the resistance of plants to pathogens.The results of this study provide a theoretical foundation for further exploring the functional mechanism of TaXI-III and discovering disease-related genes in wheat.关键词
小麦/木聚糖酶抑制剂TaXI-III/分裂泛素酵母双杂交系统/互作蛋白/超敏反应(HR)样病变诱导蛋白TaHRLI-2BKey words
wheat/xylanase inhibitor TaXI-III/split-ubiquitin yeast two-hybrid system/interacting proteins/HR-like lesion-inducing protein TaHRLI-2B分类
农业科技引用本文复制引用
刘洋,陈孝月,白裔凡,陈微,司红起,蔡华..小麦TaXI-III互作蛋白的筛选及其与TaHRLI-2B的互作分析[J].江苏农业学报,2026,42(7):1326-1337,12.基金项目
安徽省高校自然科学研究重点项目(2022AH051088) （2022AH051088）
滁州学院科研启动基金项目(2022qd50) （2022qd50）
安徽省高校自然科学研究重大项目(2023AH040223) （2023AH040223）
国家产业技术体系——小麦体系项目(CARS-03) （CARS-03）
