菌物学报2026，Vol.45Issue(7)：109-123,15.DOI:10.13346/j.mycosystema.250271
广叶绣球菌种质资源的综合评价:基于分子鉴定与表型多元统计分析
Comprehensive evaluation of germplasm resources of Sparassis latifolia based on molecular identification and multivariate statistical analysis of phenotypes
摘要
Abstract
Fifteen strains of Sparassis latifolia(10 cultivated strains and 5 wild strains)were systematically investigated.Species identity and genetic background were confirmed through molecular identification based on internal transcribed spacer(ITS)sequence analysis.Mycelial growth phenotypes and fruiting body agronomic traits of each strain were quantitatively determined.A multi-method approach including variance analysis,principal component analysis and systematic clustering analysis was employed to conduct in-depth data mining and comprehensive evaluation of the experimental results.ITS sequencing confirmed that all tested 15 strains belonged to S.latifolia.The phenotypic trait analysis revealed significant inter-strain variations in mycelial growth phenotypes and fruiting body agronomic traits.Overall,cultivated strains outperformed wild strains in fruiting body weight,biological conversion efficiency,toughness of basidiocarps,and cultivation cycle duration.Specifically,strain Sl-1 exhibited the highest basidiocarp weight[(321.07±29.18)g],while strain Sl-5 showed the fastest mycelial growth rate of the spawn[(1.13±0.04)mm/d].The basidiocarps of most cultivated strains were milky white(2A2)with moderate to good toughness,and their cultivation cycle ranged from 103 to 125 days.In contrast with cultivated strains,wild strains typically had lower yields,with yellowish basidiocarp flesh,moderate to brittle toughness,and a cultivation cycle of 108 to 132 days.A notable exception was the wild strain Sl-12,which yielded well and the yield was comparable to that of high-performing cultivated strains and displayed the highest stability(coefficient of variation=4.44%).Principal component analysis showed that accumulative contributor ration of the first three principal components was 82.47%,highlighting the main characteristic patterns and trait associations among strains.Cluster analysis categorized the 15 strains into three distinct groups:group 1 consisted entirely of cultivated strains characterized by high quality and high yield;group 2 included four cultivated strains with moderately lower yields and one wild strain;group 3 consisted of the remaining wild strains represented by Sl-12.This study provides theoretical foundation and empirical data for germplasm innovation,cultivar improvement,and industrial development of S.latifolia.关键词
广叶绣球菌/ITS序列/主成分分析/聚类分析/种质资源评价Key words
Sparassis latifolia/ITS sequence/principal component analysis/clustering analysis/germplasm resource evaluation引用本文复制引用
马云蕊,罗祥英,杨爱霞,马丽婷,周然妹,王有志,雷洪涛,李荣春..广叶绣球菌种质资源的综合评价:基于分子鉴定与表型多元统计分析[J].菌物学报,2026,45(7):109-123,15.基金项目
云南省(珍稀食用菌)企业技术中心专项资金(JSJ2023016KJ)This work was supported by the Yunnan Province(Rare Edible Fungi)Enterprise Technology Center Special Fund(JSJ2023016KJ). （珍稀食用菌）
