菌物学报2026，Vol.45Issue(7)：143-150,8.DOI:10.13346/j.mycosystema.250336
担子菌基因启动子在构巢曲霉遗传转化中的应用
Application of the gene promoters of basidiomycetes to genetic transformation of Aspergillus nidulans
摘要
Abstract
Promoters and terminators are key regulatory elements for expressing exogenous genes in genetic function studies.Due to significant genetic differences among fungal phyla,gene expression regulatory elements are generally used only within the same species or between highly similar species.Aspergillus nidulans serves as a model species for studying fungal genetic development and is also widely used in synthetic biology research for exogenous protein expression.Currently,genetic transformation in A.nidulans primarily utilizes promoters from its own constitutive gpd or tef genes,for protoplast transformation mediated by PEG.In this study,an Agrobacterium tumefaciens-mediated approach was employed to genetically transform A.nidulans apospores using the expression plasmid P020 originally developed for basidiomycetes.This plasmid contains a carboxin resistance gene expression cassette driven by the self-promoter derived from Pleurotus eryngii,and an eGFP expression cassette driven by the gpd promoter from Hypsizygus marmoreus.P.eryngii-derived carboxin gene driven by its own promoter efficiently screens positive transformants in A.nidulans via Agrobacterium tumefaciens-mediated transformation,while the gpd gene promoter from P.eryngii drives eGFP expression in A.nidulans mycelium.The above studies demonstrate that regulatory elements such as promoters and terminators from P.eryngii and H.marmoreus can successfully drive exogenous gene expression and exert protein function in A.nidulans.This study provides insights for utilizing gene regulatory elements from distantly related species in fungal expression systems.关键词
农杆菌介导转化/构巢曲霉/基因表达调控元件Key words
Agrobacterium-mediated genetic transformation/Aspergillus nidulans/gene expression regulatory element引用本文复制引用
周鑫宇,杨瑞恒,唐利华,李燕,汪滢,李玉,鲍大鹏,尚俊军..担子菌基因启动子在构巢曲霉遗传转化中的应用[J].菌物学报,2026,45(7):143-150,8.基金项目
国家自然科学基金(32172649) （32172649）
上海市"科技创新行动计划"国际科技合作项目(24390741800) （24390741800）
上海市自然科学基金(21ZR1456600)This work was supported by the National Natural Science Foundation of China(32172649),the Shanghai"Science and Technology Innovation Action Plan"International Science and Technology Cooperation Project(24390741800),and the Shanghai Natural Science Foundation(21ZR1456600). （21ZR1456600）
