临床口腔医学杂志2026，Vol.42Issue(8)：451-457,7.DOI:10.3969/j.issn.1003-1634.2026.08.002
拉曼光谱激光诱导牙本质荧光背景的物质来源及过氧化物漂白剂对其影响机制的研究
Study on the source of Raman spectral laser-induced dentin fluorescence background and its influence mecha-nism by peroxide bleaching agents
摘要
Abstract
Objective:To analyze changes in laser-induced fluorescence background following peroxide bleaching a-gent treatment using Raman spectroscopy and related techniques,identify the source of fluorescence variations.Methods:Fif-ty-four dentin sections were randomly assigned to three groups:10%neutral urea peroxide,40%neutral hydrogen peroxide,and deionized water control(n=18 each),with 24-hour reagent exposure.Raman and infrared spectroscopy were performed before and after treatment.Scanning electron microscopy examined dentin surface morphology,and non-collagen proteins were extracted for gel electrophoresis and mass spectrometry analysis.Results:Raman spectroscopy showed stable phosphate peaks while laser-induced fluorescence background intensity significantly decreased in both bleaching groups.Infrared spectroscopy demonstrated marked decline in collagen amide peaks.Scanning electron microscopy confirmed intact collagen fiber morphol-ogy.SDS-PAGE revealed marked reduction in non-collagen protein content,and mass spectrometry identified significant dif-ferential expression of non-collagen proteins associated with extracellular matrix remodeling and protein degradation.Conclu-sion:Dentin non-collagen proteins,rather than collagen,are the primary source of laser-induced fluorescence background;their oxidative degradation by peroxide bleaching agents directly reduces this background,providing molecular-level insight into tooth bleaching mechanisms.关键词
过氧化物/牙齿漂白/牙本质非胶原蛋白/拉曼光谱/质谱分析Key words
Peroxide/Teeth whitening/Dentin non-collagen/Raman spectroscopy/Mass spectrometry analysis分类
医药卫生引用本文复制引用
李锐强,尹晨,魏艺,托合提古丽·祖农,刘双慧,马琰..拉曼光谱激光诱导牙本质荧光背景的物质来源及过氧化物漂白剂对其影响机制的研究[J].临床口腔医学杂志,2026,42(8):451-457,7.基金项目
新疆维吾尔自治区自然科学基金(2022D01C265) （2022D01C265）
