皮肤性病诊疗学杂志2026，Vol.33Issue(7)：498-504,7.DOI:10.3969/j.issn.1674-8468.2026.07.003
盐酸小檗碱协同增强青霉素对产青霉素酶淋球菌的体外抗菌活性研究
Berberine hydrochloride synergistically enhances the antibacterial activity of penicillin against penicillinase producing Neisseria gonorrhoeae in vitro
摘要
Abstract
Objective This study aimed to evaluate the synergistic effect of berberine hydro-chloride on the antibacterial activity of penicillin against Penicillinase-Producing Neisseria gonor-rhoeae(PPNG),offering new insights for the prevention and treatment of highly drug-resistant PPNG.Methods The minimum inhibitory concentration(MIC)of berberine hydrochloride com-bined with penicillin against PPNG strains were determined using microbroth dilution and checker-board assays,with fractional inhibitory concentration(FIC)indices calculated to assess antibacte-rial efficacy.TEM DNA mutations were detected via gene sequencing.TEM mRNA expression levels were measured by RT-qPCR.Penicillinase was extracted by ultrasonic disruption,and its activity was assessed using the dual-wavelength colorimetric Amplite assay kit.Results The MIC range of the berberine hydrochloride-only treatment against PPNG was 0.03～128 μg/mL.The MIC of penicillin decreased by ≥4-fold and the FIC index was ≤0.5 when berberine hydrochlo-ride was combined with penicillin against PPNG,demonstrating the synergistic antibacterial effects of these two compounds.The combined antibacterial strategy did not induce mutations in TEM genes,but it decreased penicillinase activity(P＜0.05)through downregulating the mRNA ex-pression of TEM(2-ΔΔCt＜1).Conclusions Berberine hydrochloride not only directly inhibits PPNG growth but also restores penicillin sensitivity by down-regulating TEM gene expression and inhibiting penicillinase activity.These findings showed that berberine hydrochloride is an antibiotic adjuvant against PPNG.关键词
盐酸小檗碱/青霉素/产青霉素酶淋球菌/TEM基因Key words
berberine hydrochloride/penicillin/PPNG/TEM gene引用本文复制引用
唐三梅,覃晓琳,欧江丽,吴兴中,薛耀华,郑和平..盐酸小檗碱协同增强青霉素对产青霉素酶淋球菌的体外抗菌活性研究[J].皮肤性病诊疗学杂志,2026,33(7):498-504,7.基金项目
广东省中医药科研项目(20232072) （20232072）
广东省医学科研基金(A2023381,B2024182) （A2023381,B2024182）
