南华大学学报（自然科学版）2026，Vol.40Issue(2)：57-63,7.DOI:10.19431/j.cnki.1673-0062.2026.02.007
ATP在线检测设备研发、性能评估及应用研究
Research on development,performance evaluation and application of online ATP detection equipment
摘要
Abstract
Adenosine triphosphate(ATP)serves as the primary energy currency of cells,and changes in its content reflect the activity level of microorganisms.However,the lack of online detection techniques has greatly restricted the popularization and application of this indicator in relevant fields.To address this issue,this study established an online ATP detection method and developed a corresponding online ATP detection device.The performance of the instrument was evaluated in terms of its detection limit,stability and accuracy.Subsequently,this instrument was applied to two categories of biological reaction processes:a kitchen wastewater bioreactor and a Bacillus fermentation process,with ATP data obtained to analyze its correlations with pollutant removal efficiency and bacterial biomass,respectively.The results showed that the photon detection limit of the device was 174RLU～2×107 RLU;repeated measurements of ATP standard solutions at different concentrations yielded a minimum relative deviation of 3.30%,indicating good stability of the device.The detection values of the device for the same samples exhibited a linear relationship with those obtained using a microplate reader(R2=0.970 4),demon-strating high detection accuracy of the device.The ATP response trends of activated sludge in kitchen wastewater and Bacillus revealed that ATP concentration could effectively character-ize the system treatment efficiency and microbial metabolic activity.Moreover,ATP preceded bacterial biomass in indicating the entry of the microbial community into the active growth phase.关键词
ATP/微生物活性/在线检测设备/性能评估/响应趋势Key words
ATP/microbial activity/online detection equipment/performance evaluation/response trend分类
信息技术与安全科学引用本文复制引用
刘城瑜,陈杨武,余雅丹,谢雨奇,蔡王,邓钦文..ATP在线检测设备研发、性能评估及应用研究[J].南华大学学报（自然科学版）,2026,40(2):57-63,7.基金项目
四川省中试研发平台"1+N"模式合作中试项目(ZSXM2025030022) （ZSXM2025030022）
四川省科技成果转移转化引导计划项目(2023ZHJY0013) （2023ZHJY0013）
中国科学院青年创新促进会项目(2023387) （2023387）
山东得和生物技术集团有限公司委托项目(E5A2110001) （E5A2110001）
