南京中医药大学学报2026，Vol.42Issue(8)：1194-1208,15.DOI:10.14148/j.issn.1672-0482.2026.1194
银翘散加减对抽动障碍大鼠肺部菌群及外周-中枢炎症因子的影响
Effects of Modified Yinqiao San on Pulmonary Microbiota and Peripheral-Central Inflammatory Axis in Rats with Tic Disorders
摘要
Abstract
OBJECTIVE To investigate the potential mechanisms of modified Yinqiao San in treating tic disorders(TD).METHODS Forty-eight SPF-grade SD rats were randomly divided into a control group(Control,n=8)and a modeling group(n=40).An external pathogenic wind-induced TD rat model was established using 3,3′-iminodipropionitrile(IDPN)combined with alternating cold-hot stimulation.After successful modeling,the rats were re-randomized into five groups(n=8 each):model group(Model),tiapride group(Tiapride),and modified Yinqiao San high-,medium-,and low-dose groups(YQS-H,YQS-M,YQS-L).The tiapride group received 47.91 mg·kg⁻¹·d⁻¹ of tiapride solution,while the YQS-H,YQS-M,and YQS-L groups received 17.64,8.82,and 4.41 g·kg⁻¹·d⁻¹ of YQS solution,respectively,for 12 consecutive days.After the last dose,tic-like behaviors were evaluated.Patho-logical features of lung tissue and striatum were observed via HE staining.ELISA was used to measure the levels of inflammatory fac-tors TNF-α,IL-6,IL-1β,and IL-10 in bronchoalveolar lavage fluid,serum,and striatum.16S rRNA sequencing was adopted to ana-lyze microbiota structures in the upper respiratory tract(pharyngeal swabs)and lower respiratory tract(bronchoalveolar lavage fluid).RESULTS Compared to the Control group,the Model group showed increased tic behavior scores(P<0.01),elevated total movement distance and velocity,and significantly reduced resting time(P<0.01).HE staining revealed thickened alveolar walls,collapsed and deformed alveoli,interstitial pulmonary edema,and inflammatory cell infiltration.The striatal neuronal cell count decreased,with sparse and disorganized Nissl bodies.Levels of TNF-α,IL-6,and IL-1β in bronchoalveolar lavage fluid,serum,and striatum were el-evated(P<0.01),while IL-10 levels decreased(P<0.01).Compared to the Model group,the Tiapride group,YQS-H group,and YQS-M group exhibited reduced tic behavior scores,total movement distance,and velocity,along with increased resting time(P<0.05,P<0.01).Both YQS-H and YQS-M groups demonstrated significant improvements in lung tissue damage,decreased pathological scores(P<0.01),increased Nissl body counts(P<0.01),partial alleviation of disorganized arrangements,reduced levels of TNF-α,IL-6,and IL-1β in bronchoalveolar lavage fluid,serum,and striatum(P<0.01),and elevated IL-10 levels(P<0.01).The 16S rRNA results indi-cated that compared to the Control group,the relative abundance of the genus Veillonella in the upper respiratory tract was increased in the Model group(P<0.05),while the relative abundance of the genus Klebsiella in the lower respiratory tract microbiota was also el-evated(P<0.05).All YQS dosage groups were able to reduce the levels of Veillonella and Klebsiella(P<0.05).CONCLUSION Modified Yinqiao San can improve tic-like behavior in TD model rats.Its possible mechanism is related to regulating lung microbiota structure and alleviating peripheral and central inflammation.关键词
抽动障碍/银翘散/"肺-脑"轴/肺部菌群/神经炎症/炎症因子/16S rRNAKey words
tic disorders/Yinqiao San/"lung-brain"axis/pulmonary microbiota/neuroinflammation/inflammatory factors/16S rRNA分类
医药卫生引用本文复制引用
冯鹏,王鹤霖,罗瑞婷,周朝梅,陈元欢,马靖,车世杰,杨雯,李江红..银翘散加减对抽动障碍大鼠肺部菌群及外周-中枢炎症因子的影响[J].南京中医药大学学报,2026,42(8):1194-1208,15.基金项目
国家自然科学基金地区科学基金项目(82460953) （82460953）
甘肃省高校青年博士支持项目(2023QB-075) （2023QB-075）
河西学院博士科研启动经费项目(KYQD2022015) （KYQD2022015）
大学生创新创业训练计划项目(202410740035) （202410740035）
河西学院科技创新项目(2024-105) （2024-105）
