南京中医药大学学报2026，Vol.42Issue(8)：1209-1219,11.DOI:10.14148/j.issn.1672-0482.2026.1209
基于网络药理学和实验验证探讨白头翁汤干预结肠炎相关结直肠癌的作用机制
Mechanism of Baitouweng Decoction in the Treatment of Colitis-Associated Colorectal Cancer:A Study Based on Network Pharmacology and Experimental Verification
摘要
Abstract
OBJECTIVE To explore the pharmacodynamic material basis and mechanism of action of Baitouweng Decoction in the intervention of colitis-associated colorectal cancer(CAC),and to conduct preliminary verification through in vitro experiments.METHORDS Active ingredients of Baitouweng Decoction were identified using the TCMSP,Traditional Chinese Medicine Integrated Database(TCMID),and Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine(TCMIP)database com-bined with literature mining.The PharmMapper Server platform was used to predict the targets of these active ingredients.The rela-tionship between the targets of Baitouweng Decoction and CAC was verified by cross-referencing them with disease targets from Gen-eCards and drug targets associated with CAC chemotherapy from DrugBank.Network models of"Traditional Chinese Medicine-Active Ingredient-Target"and"Active Ingredient-Target"were constructed using Cytoscape software.Signaling pathways involved in the in-tervention of CAC by Baitouweng Decoction were analyzed using the DAVID database.The proliferation and apoptosis of SW480 colon cancer cells treated with Baitouweng Decoction were assessed using the CCK-8 assay and flow cytometry;the expression of inflamma-tory factors was measured via qRT-PCR;and changes in key proteins within the EGFR and PI3K/AKT signaling pathways in SW480 cells were detected by Western blot.RESULTS The"Active Ingredient-Target"network analysis identified 98 active ingredients and 87 targets(including 12 core active ingredients and 11 core targets).KEGG pathway analysis highlighted pathways related to can-cer,prostate cancer,proteoglycans in cancer,the PI3K-AKT signaling pathway,and EGFR tyrosine kinase inhibitors.Molecular dock-ing results demonstrated that compounds such as limonin,candletoxin A,and fraxin exhibited strong binding affinities with HRAS,IGF1,MAP2K1,and HSP90AA1.In vitro experiments confirmed that Baitouweng Decoction effectively inhibited the proliferation of SW480 cells and promoted apoptosis;simultaneously,it downregulated the expression of target proteins EGFR,p-AKT,and p-PI3K,and effectively suppressed the expression of IL-6,IL-1β,TNF-α,and iNOS.CONCLUSION Baitouweng Decoction inhibits the progression of colitis-associated colorectal cancer and promotes tumor cell apoptosis through a multi-level,multi-pathway mechanism involving the modulation of EGFR and its downstream signaling pathways.关键词
白头翁汤/结肠炎相关结直肠癌/网络药理学/EGFR/PI3K/AKTKey words
Baitouweng Decoction/colitis-associated colorectal cancer/network pharmacology/EGFR/PI3K/AKT分类
医药卫生引用本文复制引用
韩邢知,白雪莹,王宁,章群,孙悦鑫,钱晓萍..基于网络药理学和实验验证探讨白头翁汤干预结肠炎相关结直肠癌的作用机制[J].南京中医药大学学报,2026,42(8):1209-1219,11.基金项目
国家自然科学基金青年科学基金项目(82303970) （82303970）
南京市医学重点基金(ZKX21028) （ZKX21028）
江苏省中医药科技发展重点项目(ZD202227) （ZD202227）
江苏省自然基金面上项目(BK20211007) （BK20211007）
南京市卫生科技发展专项资金资助项目(ZDXX25125) （ZDXX25125）
