农业生物技术学报2026，Vol.34Issue(8)：1597-1611,15.DOI:10.3969/j.issn.1674-7968.2026.08.001
一个小麦种子休眠突变体的转录组和代谢组联合分析
Joint Analysis of Transcriptome and Metabolome of a Wheat(Triticum aestivum)Seed Dormancy Mutant
摘要
Abstract
The dormancy characteristics of wheat(Triticum aestivum)seeds determine its germination uniformity after sowing and regulates pre-harvest sprouting resistance of grains at harvest.In this study,the wheat mutant 1813WH,previously generated by our research team with enhanced seed dormancy and antioxidant capacity as well as decreased energy metabolism,was used as experimental material.Integrated transcriptomic and metabolomic analyses were performed on seeds at the waxy ripening stage;differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)were screened based on multi-omics data,with subsequent verification of the associated pathways.The results showed that,compared with the wild type(WT),1813WH showed altered expression of genes involved in nitrogen metabolism and differential accumulation of corresponding metabolites.Differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)were enriched in pathways including arginine synthesis,amino acid metabolism,and plant hormone signaling.The relative content of the non-reducing sugar—sucrose was significantly higher in 1813WH than in the wild type(P＜0.05),while the levels of reducing sugars including glucose-6-phosphate(G6P),fructose-1,6-bisphosphate(FBP),and fructose-6-phosphate(F6P),were significantly lower than in the wild type(P＜0.05).In addition,the relative contents of jasmonic acid(Jasmonic acid,JA)and abscisic acid(abscisic acid,ABA)were lower in 1813WH than in WT,whereas the relative cytokinin content was higher than that in WT.These results indicated that the seeds of 1813WH actively inhibited core energy metabolism pathways such as the tricarboxylic acid(TCA)cycle at the waxy ripe stage,redirecting nitrogen resources toward the synthesis of polyamines and inert nitrogen compounds such as asparagine.It was hypothesized that the 1813WH mutant might regulate the core metabolic network,alter the carbon-nitrogen metabolic balance and internal hormone levels in wheat,thereby potentially optimize its growth and stress resistance strategies.Based on this,it is speculated that seeds might maintain deep dormancy through a"resource sequestration"strategy.This study provides a new theoretical basis for subsequent elucidation of its molecular mechanism and further regulation of wheat dormancy and pre-harvest sprouting resistance.关键词
小麦突变体/氮代谢/休眠/能量代谢/氨基酸代谢Key words
Wheat mutant/Nitrogen metabolism/Dormancy/Energy metabolism/Amino acid metabolism分类
农业科技引用本文复制引用
朱莎莎,李星岩,李晓龙,韩洋,党梦圆,杨燕..一个小麦种子休眠突变体的转录组和代谢组联合分析[J].农业生物技术学报,2026,34(8):1597-1611,15.基金项目
国家自然科学基金(32460475) （32460475）
内蒙古自治区直属高校基本科研业务费(BR231519) （BR231519）
内蒙古自然科学基金重点项目(2023ZD08) （2023ZD08）
