农业生物技术学报2026，Vol.34Issue(8)：1628-1644,17.DOI:10.3969/j.issn.1674-7968.2026.08.003
陆地棉FtsH基因家族的鉴定及其在盐胁迫下的表达分析
Identification of the FtsH Gene Family in Gossypium hirsutum and Its Expression Analysis Under Salt Stress
摘要
Abstract
Filamentation temperature-sensitive H(FtsH),which possesses ATPase and proteolytic activities,is widely distributed in plants and plays important roles in plant growth and development,as well as in plant responses to stress.In this study,the function of FtsH family members in the growth,development,and salt stress response of Gossypium hirsutum were systematically explored,39 GhFtsH genes were identified in the whole genome of G.hirsutum and were analyzed using bioinformatics methods.The expression profiles of GhFtsH gene family members in various tissues were constructed using RNA-seq data.qRT-PCR was employed to detect the expression patterns of'Lumianyan 37'across distinct developmental stages under different salt concentration treatments.The results revealed that the 39 GhFtsH genes were unevenly distributed on 19 chromosomes.Phylogenetic analysis showed that 39 GhFtsH genes were divided into 7 subgroups.All GhFtsH proteins contained 2 typical conserved motifs.Synteny analysis revealed that several GhFtsH genes were collinear with Arabidopsis thaliana and rice(Oryza sativa).Promoter sequence analysis of GhFtsH identified the cis-acting elements associated with photo-response and stress responses.Most GhFtsH genes were highly expressed in leaves.Similarly,GhFtsH1,GhFtsH2,GhFtsH14,and GhFtsH15 maintained high expression levels under abiotic stress.qRT-PCR results revealed that under salt stress the expression levels of GhFtsH1,GhFtsH2 and GhFtsH14 differed with treatment concentration and developmental stage,with GhFtsH2 exhibiting the most sensitive response.This study provides an important theoretical basis for elucidating the molecular mechanisms of salt tolerance and for stress-resistance breeding in cotton.关键词
陆地棉/丝状温度敏感H蛋白酶(FtsH)/盐胁迫/生物信息学/表达模式Key words
Gossypium hirsutum/Filamentation temperature-sensitive H(FtsH)/Salt stress/Bioinformatics/Expression profile分类
农业科技引用本文复制引用
任贺丽,张乐,唐秉晖,高阳,李玉国,张军,艾尼江..陆地棉FtsH基因家族的鉴定及其在盐胁迫下的表达分析[J].农业生物技术学报,2026,34(8):1628-1644,17.基金项目
兵团科技计划(2024DA003) （2024DA003）
八师石河子市财政科技计划(2025RC05) （2025RC05）
山东省农业科学院农业科技创新工程项目(CXGC2025F03) （CXGC2025F03）
兵团科技计划项目面上项目(2025DA023) （2025DA023）
