农业生物技术学报2026，Vol.34Issue(8)：1658-1668,11.DOI:10.3969/j.issn.1674-7968.2026.08.005
辣椒CaCNGC4基因克隆及表达分析
Cloning and Expression Analysis of CaCNGC4 Gene in Pepper(Capsicum annuum)
摘要
Abstract
Cyclic nucleotide gated ion channels(CNGCs)plays an extremely important role in various physiological processes of plants,including signal transduction,growth and development,and responses to environmental stress.This study cloned the CaCNGC4 gene from peppers(Capsicum annuum)and performed bioinformatics analysis,expression pattern analysis,and subcellular localization analysis of CaCNGC4.The results showed that the CDS sequence of CaCNGC4 was 2 091 bp and encoded 696 amino acids.It was an alkaline and unstable hydrophilic protein with 6 transmembrane structures,a signal-free peptide,and the subcellular localization results showed that it was located on the plasma membrane.The CaCNGC4 protein had the highest homology with potato(Solanum tuberosum)and opium(S.dulcamara),which were 86.64%and 85.94%respectively,and the lowest homology with sweet potato(Ipomoea batatas),which was 70.07%.The expression level changes of CaCNGC4 gene under hormone(abscisic acid(ABA),gibberellin A3(GA3),methyl jasmonate(MeJA))treatment and abiotic stress(including high temperature,low temperature,and salt stress)during pepper development were detected by qPCR.The results showed that under hormone treatment and salt stress,the expression of CaCNGC4 gene first decreased,then increased,and finally decreased again.The expression of CaCNGC4 was significantly downregulated after high-temperature treatment for 1,1.5 and 3 h,while it was significantly upregulated under high-temperature treatment for 6,12 and 24 h(P＜0.05).The expression of CaCNGC4 was significantly downregulated after low-temperature treatment(P＜0.05),with no significant difference compared to the control at 24 h.The promoter region of this gene contained a series of cis-elements related to light response,hormone,external environment,and growth and development.The results of this study provide theoretical support for the regulation of pepper growth and development and the improvement of pepper varieties.关键词
辣椒/环核苷酸门控离子通道(CNGCs)/生物信息学分析/表达模式/亚细胞定位Key words
Pepper/Cyclic nucleotide gated ion channels(CNGCs)/Bioinformatics analysis/Expression pattern/Subcellular localization分类
农业科技引用本文复制引用
贾旭,黄嘉欣,郝振辛,梁燕平,王静..辣椒CaCNGC4基因克隆及表达分析[J].农业生物技术学报,2026,34(8):1658-1668,11.基金项目
山西省科技创新人才团队(202304051001018) （202304051001018）
山西省重点研发计划项目(202402140601003-01) （202402140601003-01）
山西省重点实验室开放基金课题(SCHHZDSYS2024-6) （SCHHZDSYS2024-6）
