农业生物技术学报2026，Vol.34Issue(8)：1719-1725,7.DOI:10.3969/j.issn.1674-7968.2026.08.010
红九棘鲈单拷贝基因保守序列筛选及其在种质鉴定中的应用
Screening of Single-copy Gene Conserved Sequence and Its Application in Germplasm Identification of Cephalopholis sonnerati
摘要
Abstract
The tomato hind,Cephalopholis sonnerati,is a new species of high economic value grouper,which is widely distributed,rich genetic diversity,and difficult to identify its germplasm.In order to develop molecular markers and methods suitable for germplasm identification of the tomato hind,this study used the single copy gene database of spinal animals combined with the resequencing data of the tomato hind to screen the highly conserved sequences of the tomato hind.A target sequence located in the single-copy gene ceramide glucosyltransferase(ugcg)was successfully selected,and specific primers were designed.A specific band of 226 bp with only individual base differences could be amplified from the tomato hind,but the corresponding band cannot be amplified from other groupers.In this study,a single copy conserved gene was selected as the core tool to establish a rapid and accurate identification method for the germplasm resources collection,identification and evaluation and also laid a foundation for the molecular breeding technology innovation and the artificial breeding of the tomato hind.关键词
红九棘鲈/单拷贝基因/保守序列/种质鉴定Key words
Cephalopholis sonnerati/Single copy gene/Conserved sequence/Germplasm identification分类
农业科技引用本文复制引用
刘洋,卢昇,李明,王崇伟,周波,陈有铭,宋煜,王紫苑,陈松林..红九棘鲈单拷贝基因保守序列筛选及其在种质鉴定中的应用[J].农业生物技术学报,2026,34(8):1719-1725,7.基金项目
农业生物育种重大专项(2023ZD0405506) （2023ZD0405506）
山东省重点研发计划(2023ZLYS02) （2023ZLYS02）
海南省柔性引才创新平台科研项目(YSPTZX202402) （YSPTZX202402）
海南省陈松林院士工作站(YSGZZ2023001) （YSGZZ2023001）
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2023TD20) （2023TD20）
泰山学者攀登计划 （）
青岛蓝色种业研究院蓝色种业创新项目(QDLYY-2024004) （QDLYY-2024004）
