四川轻化工大学学报（自然科学版）2026，Vol.39Issue(3)：15-22,8.DOI:10.11863/j.suse.2026.03.02
反胶束液液微萃取在亚甲基蓝检测中的应用
Reverse Micelle Mediated Dispersive Liquid-liquid Microextraction for the Detection of Methylene Blue
摘要
Abstract
Methylene blue is a water-soluble dye.Water polluted by methylene blue can induce cancer,cause environmental pollution,and lead to ecological disaster.Reverse micelle mediated dispersive liquid-liquid microextraction(RM-DLLME)is a simple,rapid and environmentally friendly novel sample preparation technique.In this study,RM-DLLME combined with spectrophotometry is used to detect methylene blue in water.After optimizing extraction conditions,for example changing the types and concentrations of surfactants,enrichment optimization is carried out to form a new method for detecting methylene blue in water by reverse micelle mediated dispersive liquid-liquid microextraction combining with spectrophotometry.Vreverse micelle solvent(3.1 mmol/L sodium dodecyl sulfate-isoamyl alcohol solution):Vmethylene blue aqueous solution(pH 4.8∶)Vmethanol=2.0∶27.5∶2.5,after vortex mixing and centrifugation,the upper phase is taken for detection at 659.0 nm.The limit of detection(LOD)and the limit of quantitation(LOQ)of the new method are 0.7 μg/mL and 2.2 μg/mL,the enrichment fator is 15.0,and the relative standard deviation(RSD)is 4.2％,meanwhile the recoveries are 90.1％～96.8％.The results show that the new method has low LOD and LOQ,good enrichment effect,small matrix effect in 5 kinds of waters,and wide application range.关键词
反胶束/液液微萃取/亚甲基蓝/分光光度法Key words
reverse micelle/dispersive liquid-liquid microextraction/methylene blue/spectrophotometry分类
化学化工引用本文复制引用
余欣忆,钟俊怡,陈燕,吴诗语,方一义,余婷婷..反胶束液液微萃取在亚甲基蓝检测中的应用[J].四川轻化工大学学报（自然科学版）,2026,39(3):15-22,8.基金项目
四川省中医药管理局科学技术研究专项课题(2024MS186) （2024MS186）
四川大学自贡市校地科技合作专项项目(2022CDZG-13) （2022CDZG-13）
四川轻化工大学大学生创新创业训练计划项目(CX2024038) （CX2024038）
