四川中医2026，Vol.44Issue(7)：48-56,9.DOI:10.26946/j.cnki.1000-3649.sczy.2601280002
基于网络药理学、分子对接与临床观察探讨痛风丸干预痛风性关节炎的作用机制
Study on the mechanisms of the Tongfeng pill in treating gouty arthritis based on network pharmacology,molecular docking and clinical observation
摘要
Abstract
Objective To investigate the potential mechanism of our hospital's empirical formula Tongfeng Pill in the treatment of gouty arthritis(GA)using network pharmacology and molecular docking,and to verify its anti-inflammatory and analgesic efficacy through clinical observation.Methods The active ingredients,core targets and pathways of the Tongfeng pill in the treatment of GA were screened by network pharmacology technology,and molecular docking was carried out using Autodock Tools.80 patients with GA were included in the clinical efficacy study.Both groups of patients received non pharmacological guidance on diet and lifestyle.The control group was treated with oral administration of Diclofenac Sodium Sustained-release Tablets 0.1g once daily,combined with external application of Dahuang Powder 10g and Jinhuang Powder 10g on the swollen and red joints for 3 days.The treatment group was additionally given Tongfeng Pill 13g orally three times daily for 3 days.The clinical efficacy,Visual Analogue Scale(VAS)score for joint pain and related inflammatory markers were analyzed before and after treatment.Results The active ingredients of Tongfeng Pill in treating GA included β-ecdysterone,rutaecarpine,Palmidin A,kaempferol and ellagic acid,etc.The core targets included TNF,BCL2,JUN,PPARG,CASP3,and SRC.KEGG enrichment mainly involved the PI3K-Akt,NF-κB,and NOD-like receptor signaling pathway.Molecular docking showed good binding affinity between core targets and active ingredients of the Tongfeng pill.Clinical observation showed that after treatment,all indicators in both groups were significantly improved compared with before treatment.However,intergroup comparison revealed no statistically significant differences in the total effective rate(95%in treatment group vs 90%in control group,P>0.05),or in the improvement of VAS score,CRP,WBC,NEU,and ESR between the two groups(P>0.05).Conclusion Tongfeng Pill exhibits multi-component,multi-target,and multi-pathway characteristics in treating GA,and its mechanism may be related to the regulation of PI3K-Akt,NF-κB,and NOD-like receptor signaling pathways and inhibition of inflammatory responses.Although the clinical observation did not demonstrate statistical advantages in conventional Western medicine inflammatory indicators,combined with the improvement trend of TCM syndromes,it suggests that Tongfeng Pill may exert therapeutic value by regulating the overall state of damp-heat accumulation pattern.关键词
痛风丸/痛风性关节炎/网络药理学/分子对接/临床观察Key words
Tongfeng pill/Gouty arthritis/Network pharmacology/Molecular docking/Clinical observation分类
医药卫生引用本文复制引用
夏海越,谢涛,胡斌,廖晓莉,陈艳英..基于网络药理学、分子对接与临床观察探讨痛风丸干预痛风性关节炎的作用机制[J].四川中医,2026,44(7):48-56,9.基金项目
四川省中医药管理局中医药科研专项面上项目(2024MS472). （2024MS472）
