山东农业科学2026，Vol.58Issue(7)：86-95,10.DOI:10.14083/j.issn.1001-4942.2026.07.010
褪黑素对低氮胁迫下烤烟幼苗生理特性的影响
Influence of Melatonin on Physiological Characteristics of Flue-Cured Tobacco Seedlings under Low Nitrogen Stress
摘要
Abstract
To explore the effect of melatonin on physiological characteristics of flue-cured tobacco seed-lings under low nitrogen stress,the experiment was carried out by using flue-cured tobacco varieties 9A05 and 9K30 as experimental materials,and setting four treatments of CK(the control,4 mmol·L-1 NO-3+0 μmol·L-1 melatonin),CKM(4 mmol·L-1 NO-3+100 μmol·L-1 melatonin),LN(0.4 mmol·L-1 NO-3+0 μmol·L-1 me-latonin)and LNM(0.4 mmol·L-1 NO-3+100 μmol·L-1 melatonin).The effects of foliar spray of melatonin on the morphological characteristics,photosynthetic performance,osmotic regulatory and nitrogen utilization of flue-cured tobacco seedlings under low nitrogen stress were analyzed.The results were as follows.(1)Spraying melatonin could alleviate the inhibition of low nitrogen stress on the growth of flue-cured tobacco seedlings.Compared with LN,the LNM treatment increased the plant height,stem circumference,leaf area,fresh weight of above-ground part and root fresh weight of 9A05 by 40.61%,52.83%,17.86%,27.58%and 34.06%respectively,and most reached significant level.(2)Spraying melatonin could increase the SPAD value of flue-cured tobacco seedlings under low nitrogen stress.Compared with LN,the LNM treatment signifi-cantly increased the SPAD value of 9K30 by 15.53%,and it also had a certain promoting effect on 9A05 with an increase of 2.68%.(3)Spraying melatonin could enhance the photosynthetic characteristics of flue-cured tobacco seedlings under low nitrogen stress.Compared with LN,the LNM treatment increased the net photo-synthetic rate(Pn)of the varieties 9A05 and 9K30 with the increase of 17.12%and 23.37%,respectively.(4)Spraying melatonin could enhance the nitrogen metabolism of flue-cured tobacco seedlings under low ni-trogen stress.Compared with LN,the LNM treatment increased the content of nitrate nitrogen in the leaves and roots of seedlings,and improved the activity of nitrate reductase in the leaves.(5)Spraying melatonin could affect the content of osmotic regulatory substances in flue-cured tobacco seedlings under low nitrogen stress.The contents of soluble protein and proline decreased,while the content of soluble sugar increased.(6)Through correlation analysis and principal component analysis based on the tested 18 physiological indicators of the two varieties,followed by a comprehensive analysis,it was concluded that the LNM treatment was superior to the LN treatment for the two varieties.In conclusion,spraying melatonin could alleviate the low nitrogen stress to flue-cured tobacco seedlings and promote their growth.关键词
烤烟/低氮胁迫/褪黑素/生理特性Key words
Flue-cured tobacco/Low nitrogen stress/Melatonin/Physiological characteristics分类
农业科技引用本文复制引用
孙寿杰,霍佳琪,王平平,艾绥龙,张立新..褪黑素对低氮胁迫下烤烟幼苗生理特性的影响[J].山东农业科学,2026,58(7):86-95,10.基金项目
中国烟草总公司陕西省公司科技项目(KJ-2024-01) （KJ-2024-01）
