兽类学报2026，Vol.46Issue(4)：575-585,11.DOI:10.16829/j.slxb.151080
基于全基因组重测序的辽宁海域东亚江豚遗传特性研究
Genetic characteristics of Neophocaena sunameri in Liaoning coastal wa-ters based on whole genome resequencing
摘要
Abstract
In order to better understand the population structure and genetic differences of the East Asian finless porpoise(Neophocaena sunameri)distributed in Liaoning coastal waters,and to provide scientific basis for species conservation,this study collected muscle tissues from 47 individuals of the northern population of N.sunameri(NSN)from Liaoning coastal waters for whole genome resequencing,with a sequencing depth of 10 ×.Subsequently,the sequence data of fin-less porpoises from the southern population of N.sunameri(NSS)from the Yangtze River estuary,the population of N.asiaeorientalis(NA)from the Yangtze River basin,and the population of N.phocaenoides(NP)from the Fujian sea area were downloaded from the NCBI database.Genetic diversity analysis,genetic structure analysis,principal component analysis,phylogenetic tree construction,genetic differentiation index,gene flow,and genetic distance analysis were con-ducted using sequencing data from the four populations.The observed heterozygosity(Ho)and expected heterozygosity(He)of the population in Liaoning coastal waters were 0.312 3 and 0.321 0,respectively,indicating that the genetic di-versity of the finless porpoise distributed in the Liaoning oastal waters is not low and represents a large and stable popula-tion.The results of phylogenetic tree analysis,principal component analysis,and genetic structure analysis were consis-tent,all of which can distinguish different populations.Overall,the four populations can be divided into three groups.Group Ⅰ includes NP population,Group Ⅱ represents NA population and Group Ⅲ represents the East Asian finless por-poise population,including the MSN and NSS populations.The genetic differentiation index between NSN and NP was 0.178 0,the genetic differentiation index between NSN and NA was 0.047 8,the genetic differentiation index between NSN andNSS was 0.023 1,the genetic distance was 0.031 9,and the gene flow was 10.554 5.In summary,East Asian finless porpoises distributed in the Liaoning coastal waters exhibit relatively high genetic diversity.They belong to the same species as NSS,with sufficient gene exchange and low genetic differentiation,and can be protected as a manage-ment unit.关键词
全基因组重测序/东亚江豚/群体结构/遗传差异/辽宁海域Key words
Whole genome resequencing/Neophocaena sunameri/Population structure/Genetic differentiation/Liaon-ing coastal waters分类
生物科学引用本文复制引用
高祥刚,王震,鹿志创,杜静,邢衍阔,韩家波,田甲申..基于全基因组重测序的辽宁海域东亚江豚遗传特性研究[J].兽类学报,2026,46(4):575-585,11.基金项目
中国海油海洋环境与生态保护公益基金(CF-MEEC/TR/2024-3) （CF-MEEC/TR/2024-3）
