饲料研究2026，Vol.49Issue(12)：25-30,6.DOI:10.13557/j.cnki.issn1002-2813.2026.12.005
溶菌酶二聚体对产肠毒素大肠杆菌攻毒断奶仔猪生长性能、肠道形态和肠道微生物菌群结构的影响
Effects of lysozyme dimer on growth performance,intestinal morphology,and intestinal microbiota structure of weaned piglets challenged with enterotoxigenic Escherichia coli
摘要
Abstract
The experiment aimed to investigate the effects of lysozyme dimer on the growth performance,organ indices,serum biochemical indices,intestinal mucosal antioxidant capacity,intestinal morphology,and intestinal microbiota counts of weaned piglets before and after challenge with enterotoxigenic Escherichia coli(ETEC).A total of 400 healthy DLY(Duroc×Landrace×Yorkshire)weaned piglets with an initial body weight of(6.82±0.10)kg were randomly divided into four groups,with 20 replicates per group and five piglets per replicate.The control group(group CON)was fed a basal diet,the group L was fed the basal diet+1 500 mg/kg lysozyme dimer,the group ET was fed the basal diet+orally administered with 20 mL of 0.01%Escherichia coli broth,the group LE was fed the basal diet+1 500 mg/kg lysozyme dimer+orally administered with 20 mL of 0.01%Escherichia coli broth.The pre-test period was three days,and the formal test period was 14 days.The results showed that compared with the group CON,the relative weights of the duodenum and jejunum in the group ET were significantly decreased(P<0.05).Compared with group ET,the relative weights of the duodenum and jejunum in the group LE were significantly increased(P<0.05).Compared with the group CON,the serum total protein(TP)and globulin(GLB)concentrations in the group L were significantly decreased(P<0.05),while the albumin/globulin(A/G)was significantly increased(P<0.05).Compared with the group ET,the serum TP and GLB concentrations in the group LE were significantly decreased(P<0.05),while the A/G was significantly increased(P<0.05).Compared with the group CON,the villus height to crypt depth ratios of the jejunum and ileum in the group L were significantly increased(P<0.05).Compared with the group ET,the villus height to crypt depth ratios of the jejunum and ileum in the group LE were significantly increased(P<0.05).Compared with the group CON,the number of Bifidobacterium in the ileum of the group ET was significantly decreased(P<0.05).Compared with the group ET,the number of Bifidobacterium in the ileum,as well as the numbers of Lactobacillus and Bifidobacterium in the colon of the group LE were significantly increased(P<0.05),while the number of Escherichia coli in the colon was significantly decreased(P<0.05).The study shows that dietary supplementation with 1 500 mg/kg lysozyme dimer can improve serum biochemical indices and intestinal morphology,and maintain intestinal microbiota homeostasis in weaned piglets.Moreover,lysozyme dimer can alleviate the disruption of intestinal morphology and microbial balance induced by ETEC challenge,thereby protecting the intestinal health of weaned piglets.关键词
溶菌酶二聚体/断奶仔猪/腹泻/器官指数/肠道微生物/产肠毒素大肠杆菌Key words
lysozyme dimer/weaned piglets/diarrhea/organ index/intestinal microbiota/enterotoxigenic Escherichia coli分类
农业科技引用本文复制引用
张容恺,吕周亚,崔光亚,李换,代张超,胡媛,吴秋珏,马岩..溶菌酶二聚体对产肠毒素大肠杆菌攻毒断奶仔猪生长性能、肠道形态和肠道微生物菌群结构的影响[J].饲料研究,2026,49(12):25-30,6.基金项目
国家自然科学基金青年基金项目(项目编号:32302742) （项目编号:32302742）
河南科技大学博士科研启动专项(项目编号:13480086) （项目编号:13480086）
