饲料研究2026，Vol.49Issue(12)：50-56,7.DOI:10.13557/j.cnki.issn1002-2813.2026.12.009
嗜吡啶红球菌发酵黄芪对石斑鱼生长性能、消化、免疫和抗氧化指标的影响
Effects of Rhodococcus pyridinivorans fermented Astragalus on growth performance,digestion,immune,and antioxidant indicators of grouper
摘要
Abstract
The experiment aimed to investigate the effects of dietary supplementation with Rhodococcus pyridinivorans fermented Astragalus on the growth performance,immune function,digestive enzyme activities,and antioxidant indicators of pearl gentian grouper.A total of 360 pearl gentian groupers with a body weight of(9.56±1.14)g were selected and randomly divided into four groups,with three replicates per group,and 30 fish per replicate.The control group was fed a basal diet,while groups T1,T2 were fed the basal diet supplemented with 1%Astragalus powder,1%Rhodococcus pyridinivorans fermented Astragalus powder,respectively.The feed in group T3 was sprayed with 1%Rhodococcus pyridinivorans cell suspension.After the standard rearing period,the fish were challenged with 1×108 CFU/mL of Vibrio harveyi for 10 days,with the injection volume being 1%of the fish's body weight.The preliminary trial lasted seven days,followed by 28 days of standard rearing and a 10-day challenge test period.The results showed that the weight gain rate and specific growth rate in the group T2 were significantly higher than those in the group T3 and the control group(P<0.05),while the feed coefficient was significantly lower than that in the other groups(P<0.05).The hepatic activities of acid phosphatase(ACP)and alkaline phosphatase(AKP),as well as muscle lysozyme(LZM)activity in the groups T1 and T2 were significantly higher than those in the other groups(P<0.05).The serum activities of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in the group T2 were significantly lower than those in the group T3(P<0.05).The intestinal activities of lipase,pepsin,trypsin,and α-amylase in the group T2 were significantly higher than those in the other groups(P<0.05).The hepatic peroxidase(POD)activity,total antioxidant capacity(T-AOC),catalase(CAT)activity,and glutathione(GSH)content in the groups T1 and T2 were significantly higher than those in the other groups(P<0.05),while the muscle malondialdehyde(MDA)content in the group T2 was significantly lower than that in the other groups(P<0.05).The challenge test results showed that the survival rate in the group T2 reached 100.00%,and the hepatic non-specific immune and antioxidant indicators were significantly better than those in the other groups(P<0.05).The study shows that dietary supplementation with Rhodococcus fermented Astragalus can effectively enhance the immune function,digestive enzyme activities,and antioxidant capacity of pearl gentian grouper,promote healthy growth,and strengthen their resistance against Vibrio harveyi infection.关键词
发酵黄芪/珍珠龙胆石斑鱼/抗氧化/非特异性免疫/生长性能/消化酶活性/哈维氏弧菌Key words
fermented Astragalus/pearl gentian grouper/antioxidation/non-specific immunity/growth performance/digestive enzyme activity/Vibrio harveyi分类
农业科技引用本文复制引用
崔景婷,蔡伟霖,于宏,宋涛,张海光,杨晓晗,方珍珍,孙学亮..嗜吡啶红球菌发酵黄芪对石斑鱼生长性能、消化、免疫和抗氧化指标的影响[J].饲料研究,2026,49(12):50-56,7.基金项目
甘肃省科技厅东西部协作(津甘合作)专项(项目编号:23CXND0002、25CXNE014) （津甘合作）
津甘双地科技特派员项目(项目编号:24CXNE025) （项目编号:24CXNE025）
河北省农业科技成果转化资金项目(项目编号:2025JNZ-J03) （项目编号:2025JNZ-J03）
天津市科技计划项目(项目编号:23ZYCGSN00780) （项目编号:23ZYCGSN00780）
天津市海水养殖产业技术体系创新团队项目(项目编号:ITTMRS2026000) （项目编号:ITTMRS2026000）
