宿州学院学报2026，Vol.41Issue(6)：23-28,6.DOI:10.3969/j.issn.1673-2006.2026.06.005
小麦TrhemA基因克隆及生物信息学分析
Cloning and Bioinformatics Analysis of TrhemA Gene in Wheat
摘要
Abstract
Structural and functional studies of glutamyl-tRNA reductase(GluTR)are crucial for understanding the regulatory mechanism of chlorophyll synthesis.To investigate the expression pattern and function of GluTR in wheat,the TrhemA gene was cloned using RT-PCR technology and subjected to bioinformatics analysis in this study.The results indicated that the open reading frame(ORF)of TrhemA is 1 479 bp in length,encoding a protein composed of 492 amino acids.The deduced TrhemA protein contains six domains,including HemA and other characteristic do-mains,and is an unstable hydrophilic protein without a signal peptide.A total of seven functional sites were identi-fied,such as N-glycosylation,protein kinase-C phosphorylation,and casein kinase Ⅱ phosphorylation sites,among others.Protein structure prediction revealed that the TrhemA protein may localize to both the chloroplast and the cy-toplasm,and its structure is primarily composed of α-helix and random coil.The wheat GluTR(TraesCS7A02G1256000)shares 91.46％amino acid sequence identity with that of Aegilops tauschii(XP_045087689.1),and has the closest genetic relationship.This study successfully cloned the TrhemA gene from wheat and performed a comprehensive bioinformatics analysis,providing a theoretical foundation for further research into its molecular mechanism.关键词
小麦/谷氨酰-tRNA还原酶/基因克隆/生物信息学分析Key words
wheat/glutamyl-tRNA reductase/gene cloning/bioinformatics analysis分类
生物科学引用本文复制引用
姜颖,邓娇,张青霞,王静,安淑芬,李静..小麦TrhemA基因克隆及生物信息学分析[J].宿州学院学报,2026,41(6):23-28,6.基金项目
安徽省高等学校科学研究项目(2024AH051823) （2024AH051823）
宿州学院博士(后)科研启动基金项目(2022BSK036) （后）
宿州学院资助大学生创新创业训练计划项目(S202410379091). （S202410379091）
