枣胚胎发育蛋白质分析及氨基酸代谢通路关键蛋白筛选
Proteome Analysis and Amino Acid Metabolism Pathway of Jujube Embryo
摘要
Abstract
Embryo dysplasia or abortion is a prevalent disorder in jujube(Ziziphus jujuba Mill.)em-bryos is a common phenomenon,which seriously restricts jujube cross breeding.Proteins are closely related to embryo development.Currently,systematic analyses of proteins involved in amino acid me-tabolism during embryo development in jujube varieties with different fertility levels are lacking.To explore the protein composition and amino acid expression differences in jujube embryos at different developmental stages,this study employed data independent acquisition(DIA)technology to perform Gene Ontology(GO)functional annotation of differential proteins,Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathway annotation,and cluster analysis of highly embryo-fertile'Sour jujube'(Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chow)and low embryo fertility cul-tivar'Kongfusucui jujube'at three different developmental stages.The results showed that a total of 10 516 proteins were identified,among which 1 558 proteins exhibited significant differential expres-sion.Among these differential proteins,1 337 had catalytic activity,and 261 proteins were involved in amino acid synthesis and metabolism.GO enrichment analysis revealed that the differentially ex-pressed proteins with catalytic activity were mainly enriched at 30 and 50 days after embryo formation.The expression levels of serine acetyltransferase(XP_015865688.1),serine hydroxymethyltransferase(XP_015868935.1),and 3-phosphoshikimate 1-carboxyvinyltransferase(XP_015888682.1),which are involved in amino acid metabolism,showed a continuous increase during embryo development in em-bryo-fertile'Sour jujube',while in embryos of the low-fertility cultivar'Kongfusucui jujube',their expression first decreased and then increased.In particular,serine acetyltransferase(XP_015865688.1)was significantly down-regulated at the peak of abortion(40 days after embryo formation)during the KD4 vs.SD4 period,suggesting that it plays a key role in regulating amino acid accumulation.关键词
枣/胚发育/蛋白组/氨基酸/DIAKey words
Ziziphus jujuba Mill./Embryo development/Proteomics/Amino acid/Data independ-ent acquisition引用本文复制引用
马晴,李毅,杨梦帆,任海燕,王永康,赵爱玲,薛晓芳,苏万龙,石美娟,刘丽..枣胚胎发育蛋白质分析及氨基酸代谢通路关键蛋白筛选[J].西北农业学报,2026,35(8):1460-1469,10.基金项目
山西省科技重大专项计划"揭榜挂帅"项目(202201140601027) （202201140601027）
山西省自然科学基金(202103021224147) （202103021224147）
农业农村部种质资源保护专项(111721301354052002) （111721301354052002）
国家科技资源共享服务平台项目(NHGRC2023-NH12-1) （NHGRC2023-NH12-1）
现代农业产业技术体系专项(CARS-30-1-07,CARS-30-ZZ-22). The Major Science and Technology Special Project of Shanxi Province under the"Unveiling the List and Taking the Lead"Program(No.202201140601027) （CARS-30-1-07,CARS-30-ZZ-22）
the Natural Science Foundation of Shanxi Province(No.202103021224147) （No.202103021224147）
the Special Project for Germplasm Resources Conservation of the Ministry of Agriculture and Rural Affairs(No.111721301354052002) （No.111721301354052002）
the Project of the National Science and Technology Resources Sharing Service Platform(No.NHGRC2023-NH12-1) （No.NHGRC2023-NH12-1）
the Special Project of the Modern Agricultural Industry Technology System(No.CARS-30-1-07,No.CARS-30-ZZ-22). （No.CARS-30-1-07,No.CARS-30-ZZ-22）
