中草药2026，Vol.57Issue(14)：5470-5475,6.DOI:10.7501/j.issn.0253-2670.2026.14.009
香港巨牡蛎X射线衍射分析及指纹图谱建立
X-ray diffraction analysis and fingerprint establishment of Crassostrea hongkongensis
摘要
Abstract
Objective To establish X-ray diffraction(XRD)fingerprint profiles of Crassostrea hongkongensis and determine its calcium carbonate content.Methods A total of 16 batches of C.hongkongensis samples were analyzed by XRD to establish the fingerprint.Based on hierarchical clustering analysis(HCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA),characteristic information for identification of Crassostrea Hongkongensis concha was screened with variable importance in projection(VIP)value>1.The content of calcium carbonate in the samples was determined by EDTA complexometric titration.Results The XRD fingerprint of C.hongkongensis was established,and its phase structure belongs to calcite-type calcium carbonate,with 13 common peaks identified.The similarity of all 16 batches of samples was greater than 0.99.HCA divided the 16 batches into 3 categories,and the VIP values of OPLS-DA showed that 5 diffraction angles made great contributions,which were 47.41°,65.51°,61.28°,47.04° and 57.34° respectively.Among the 16 batches of samples,15 batches had a calcium carbonate content exceeding 94%.Conclusion The established XRD fingerprint can be used for the identification and analysis of C.hongkongensis.Their quality meets the standards specified under Ostreae Concha recorded in the Pharmacopoeia of the People's Republic of China(2025 edition),offering technical support for their development as traditional Chinese medicinal material and comprehensive utilization.关键词
香港巨牡蛎/X 射线衍射/指纹图谱/相似度/碳酸钙Key words
Crassostrea hongkongensis Dunker/X-ray diffraction/fingerprint/similarity/calcium carbonate分类
医药卫生引用本文复制引用
米顺利,李琪琦,陈艺萍,陈美娟,高程海,易湘茜..香港巨牡蛎X射线衍射分析及指纹图谱建立[J].中草药,2026,57(14):5470-5475,6.基金项目
中药壮瑶药创新药物教育部工程研究中心经费资助(ZYZYY2025036) （ZYZYY2025036）
广西海洋药物重点实验室能力强化建设项目资助(桂科LT2600640033) （桂科LT2600640033）
2026年广西向海经济人才培养支持专项 （）
广西青年岐黄学者培养项目(GXQH202412) （GXQH202412）
广西中医药大学岐黄工程高层次人才团队培育项目(202404) （202404）
广西重点研发计划项目(桂科 AB25069014) （桂科 AB25069014）
广西中医药大学研究生教育创新计划项目(YCSY2023066) （YCSY2023066）
