中国临床药理学杂志2026，Vol.42Issue(12)：1703-1709,7.DOI:10.13699/j.cnki.1001-6821.2026.12.009
葛根素调控SDF-1/CXCR4通路改善压力性尿失禁大鼠排尿功能的研究
Research of puerarin improving the urination function of rats with stress urinary incontinence by adjusting the SDF-1/CXCR4 pathway
摘要
Abstract
Objective To investigate the effect of puerarin(PU)on the urination function of rats with stress urinary incontinence(SUI)by adjusting the stromal cell-derived factor-1(SDF-1)/CXC chemokine receptor 4(CXCR4)pathway and to explore its mechanism of action.Methods A rat model of SUI was established by bilateral oophorectomy combined with vaginal dilation.After successful modeling,the rats were randomly divided into the model group,low dose-experimental group,high dose-experimental group,the positive control group,the activator group and the inhibitor group,10 rats in each group.Additionally,10 normal rats were selected as the blank control group.Rats in the low and high dose-experimental groups were administered 17.5 and 70.0 mg·kg-1 PU by gavage,respectively.Rats in the positive control group were administered 20.0 mg·kg-1 midodrine by gavage.Rats in the activator group were injected with 10.0 mg·kg-1 pathway activator CTCE-0214 via the tail vein.Rats in the inhibitor group were administered 70.0 mg·kg-1 PU by gavage combined with intraperitoneal injection of 5.0 mg·kg-1 pathway inhibitor AMD3100.The sneezing positive rate was evaluated by mechanical stimulation method,urodynamic indicators[maximum bladder capacity(MBC),abdominal leak point pressure(ALPP)and leak point pressure(LPP)]were determined by cystometrography,pelvic floor muscle contractility/muscle weight ratio and urination function indicators(urine output,residual urine volume and voiding efficiency)were evaluated by electrophysiological method,pathological changes of urethral tissue by hematoxylin-eosin(HE)and Masson staining,and the relative expression levels of SDF-1/CXCR4 pathway-related proteins in urethral tissue were determined by Western blotting.Results The urodynamic indicator MBC in the blank control group,model group,low dose-experimental group,high dose-experimental group,the positive control group,the activator group and the inhibitor group were(6.57±0.81),(1.36±0.17),(1.95±0.24),(5.83±0.62),(5.64±0.58),(5.72±0.60)and(2.49±0.35)mL,respectively;the ALPP was(45.76±4.83),(11.52±1.49),(17.68±1.95),(41.25±4.37),(39.06±4.16),(40.65±4.23)and(18.97±2.44)mmHg,respectively;the LPP was(43.29±5.08),(9.76±1.35),(14.58±1.63),(39.62±4.59),(37.05±3.92),(38.26±4.25)and(18.43±2.16)mmHg,respectively;the pelvic floor muscle contractility/muscle weight ratio was 4.03±0.42,1.06±0.15,1.79±0.18,3.85±0.43,3.62±0.37,3.73±0.39 and 1.94±0.21,respectively;the voiding efficiency was(96.24±3.76)％,(41.65±5.83)％,(58.39±6.45)％,(89.46±8.26)％,(85.12±9.04)％,(87.14±8.59)％and(64.95±6.72)％,respectively;the relative expression levels of SDF-1 protein was 0.99±0.12,0.42±0.06,0.58±0.08,0.91±0.09,0.86±0.09,0.88±0.09 and 0.63±0.07,respectively;the relative expression levels of CXCR4 protein was 1.02±0.11,0.37±0.04,0.46±0.06,0.93±0.12,0.89±0.11,0.91±0.12 and 0.49±0.05,respectively;the phosphorylated protein kinase B/protein kinase B(p-AKT/AKT)ratio was 0.90±0.09,0.28±0.03,0.52±0.06,0.86±0.09,0.81±0.07,0.83±0.08 and 0.43±0.05,respectively.Comparisons between the blank control group and the model group,between the model group and the low dose-,high dose-experimental group,positive group or activator group,and between the high dose-experimental group and the inhibitor group all showed statistically significant differences(all P＜0.05).Conclusion PU can improve the urination function of rats with SUI,and this may be related to the activation of the SDF-1/CXCR4 pathway.关键词
葛根素/压力性尿失禁/基质细胞衍生因子-1/CXC趋化因子受体4通路/排尿功能Key words
puerarin/stress urinary incontinence/matrix cell-derived factor-1/cxc chemokine receptor 4 pathway/urination function分类
医药卫生引用本文复制引用
陈嫣,杨晨,叶利群..葛根素调控SDF-1/CXCR4通路改善压力性尿失禁大鼠排尿功能的研究[J].中国临床药理学杂志,2026,42(12):1703-1709,7.基金项目
国家中医药管理局科技司——浙江省中医药管理局共建项目(编号:GZY-ZJ-KJ-Z3090) （编号:GZY-ZJ-KJ-Z3090）
