中国美容医学2026，Vol.35Issue(8)：1-7,7.
大鼠颌骨和股骨骨髓来源间充质干细胞生物学特性的对比研究
Comparison of Biological Characteristics of Mesenchymal Stem Cells Derived from Rat Orofacial and Femur Marrow
摘要
Abstract
Objective To explore the differences in biological characteristics between rat orofacial derived mesenchymal stem cells(OMSCs)and bone marrow mesenchymal stem cells(BMMSCs),aiming to provide higher quality seed cells for bone tissue engineering.Methods OMSCs and BMMSCs were extracted from the mandibles and femurs of SD rats,respectively.The surface marker molecules were identified by flow cytometry.Differences in cell proliferation,migration,and osteogenic differentiation between OMSCs and BMMSCs under titanium plate stimulation were compared using the MTT assay,scratch test,RT-PCR,and alizarin red staining.The ability of bone tissue formation in vivo was assessed by heterotopic ossification experiments in nude mice.Results Both OMSCs and BMMSCs met the characteristics of mesenchymal stem cells.However,compared with BMMSCs,OMSCs exhibited a stronger cell proliferation rate and migration ability under titanium plate stimulation.The OMSCs group formed more mineralized nodules,with higher expression of osteogenic-related genes Runx2,ALP,and OCN.The heterotopic ossification experiments further showed that the new bone formation in the OMSCs group was significantly more than that in the BMMSCs group,indicating that OMSCs had a significantly higher osteogenic differentiation capacity than BMMSCs.Conclusion OMSCs exhibit superior biological characteristics which can provide higher-quality seed cells for bone tissue regeneration engineering.关键词
骨组织工程/种子细胞/颌骨来源间充质干细胞/细胞迁移/成骨分化Key words
bone tissue engineering/seed cell/orofacial derived mesenchymal stem cells/cell migration/osteogenic differentiation分类
医药卫生引用本文复制引用
高奕莉,王莉,孟庆悦,杨茂林,程兵坤,程子绪..大鼠颌骨和股骨骨髓来源间充质干细胞生物学特性的对比研究[J].中国美容医学,2026,35(8):1-7,7.基金项目
河北省自然科学基金(编号:H2020109157) （编号:H2020109157）
廊坊市科技局项目(编号:2021013110) （编号:2021013110）
